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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

CO2 and NH3
Lactate and ammonium
Lactate only
CO2 and lactate

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

primary cell lines
established cell lines
propagated cell lines
secondary cell lines

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

no change in pH
no loss of cell viability
increase in pH
reduction in the pH of culture hence loss of cell viability

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

physical disruption
All of these
treating with chelating agents
enzymatic digestion

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

It participates in the de novo synthesis of purines and pyrimidines
All of these
Helps in the cells respiration
For monitoring pH of the culture

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

increase in specific lactate production from glucose
accumulation of ammonia
both (a) and (b)
incomplete glutamine oxidation

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