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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

Lactate only
Lactate and ammonium
CO2 and lactate
CO2 and NH3

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

75-105 mmol/litre
55 - 75 mmol/litre
105-150 mmol/litre
5.5 - 55 mmol/litre

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

280-360
150-300
360-400
300-325

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

both (a) and (b)
accumulation of ammonia
incomplete glutamine oxidation
increase in specific lactate production from glucose

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Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it forms a semi solid coagulum
it forms a clear and solid coagulum even after dilution
it doesn't produce solid clots
it is too opaque

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

Helps in the cells respiration
It participates in the de novo synthesis of purines and pyrimidines
All of these
For monitoring pH of the culture

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