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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

Lactate and ammonium
CO2 and NH3
Lactate only
CO2 and lactate

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

They act as growth inhibitors
They act as growth promoters
Have no effect on cells
Lactate helps in the growth while ammonium inhibits the growth

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

1-2 mmol/litre
2-7 mmol/litre
7-15 mmol/litre
15 - 20 mmol/litre

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Lactic acid
Glucose
Creatine phosphate
ATP

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

no loss of cell viability
increase in pH
no change in pH
reduction in the pH of culture hence loss of cell viability

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Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Slide culture
Adherent primary culture
Carrel flask culture
Roller test tube culture

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