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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

CO2 and NH3
Lactate and ammonium
Lactate only
CO2 and lactate

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

Lactate helps in the growth while ammonium inhibits the growth
They act as growth inhibitors
They act as growth promoters
Have no effect on cells

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

metabolism of nutrients in culture media at a rate greater than that required for consumption
lipid peroxidation
All of these
DNA degradation

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

It participates in the de novo synthesis of purines and pyrimidines
All of these
Helps in the cells respiration
For monitoring pH of the culture

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

anaerobic pathway
both (a) and (b)
none of these
oxidative pathway

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

stable
unstable
anchorage dependent
anchorage independent

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