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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

Lactate and ammonium
CO2 and NH3
CO2 and lactate
Lactate only

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

anchorage independent
stable
anchorage dependent
unstable

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

lipid peroxidation
DNA degradation
metabolism of nutrients in culture media at a rate greater than that required for consumption
All of these

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

enzymatic digestion
physical disruption
All of these
treating with chelating agents

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

All of these
by removal of ammonia or ammonium from culture medium
by controlled addition of glutamine at low level
by substituting glutamine by glutamate

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Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

8 amino acids
10 amino acids
6 amino acids
12 amino acids

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