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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

7-15 mmol/litre
2-7 mmol/litre
15 - 20 mmol/litre
1-2 mmol/litre

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Glucose
Lactic acid
Creatine phosphate
ATP

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by substituting glutamine by glutamate
All of these
by removal of ammonia or ammonium from culture medium
by controlled addition of glutamine at low level

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Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Slide culture
Carrel flask culture
Roller test tube culture
Adherent primary culture

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

5 - 10 pmol of O2/cell/h
0.05-5 nmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
0.05-5 pmol of O2/cell/h

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Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Cytokines
Growth factors
All of these
Glucose and Glutamine

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