Animal Cell Culture and Regulation Range of optimum glutamine concentration present in the culture media is 1-2 mmol/litre 15 - 20 mmol/litre 2-7 mmol/litre 7-15 mmol/litre 1-2 mmol/litre 15 - 20 mmol/litre 2-7 mmol/litre 7-15 mmol/litre ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation An established cell line can be called where it has been subcultured at least 70 times at an interval of 1 day between subcultures 50 times at an interval of 3 days between subcultures 70 times at an interval of 3 days between subcultures 40 times at an interval of 3 days between subcultures 70 times at an interval of 1 day between subcultures 50 times at an interval of 3 days between subcultures 70 times at an interval of 3 days between subcultures 40 times at an interval of 3 days between subcultures ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of increase in specific lactate production from glucose incomplete glutamine oxidation both (a) and (b) accumulation of ammonia increase in specific lactate production from glucose incomplete glutamine oxidation both (a) and (b) accumulation of ammonia ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation How does CO2 help in the cell metabolism during cell culture? Helps in the cells respiration All of these It participates in the de novo synthesis of purines and pyrimidines For monitoring pH of the culture Helps in the cells respiration All of these It participates in the de novo synthesis of purines and pyrimidines For monitoring pH of the culture ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation To prevent the accumulation of lactate low glucose concentration is required high glucose concentration is required high glutamine concentration is required low glutamine concentration is required low glucose concentration is required high glucose concentration is required high glutamine concentration is required low glutamine concentration is required ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Toxicity due to accumulation of ammonia can be overcome by removal of ammonia or ammonium from culture medium by substituting glutamine by glutamate All of these by controlled addition of glutamine at low level by removal of ammonia or ammonium from culture medium by substituting glutamine by glutamate All of these by controlled addition of glutamine at low level ANSWER DOWNLOAD EXAMIANS APP