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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

1-2 mmol/litre
15 - 20 mmol/litre
2-7 mmol/litre
7-15 mmol/litre

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Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it doesn't produce solid clots
it is too opaque
it forms a semi solid coagulum
it forms a clear and solid coagulum even after dilution

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

secondary cell lines
established cell lines
propagated cell lines
primary cell lines

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

incomplete glutamine oxidation
increase in specific lactate production from glucose
both (a) and (b)
accumulation of ammonia

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

Have no effect on cells
Lactate helps in the growth while ammonium inhibits the growth
They act as growth promoters
They act as growth inhibitors

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Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

both (a) and (b)
release of genetic information
phenotypic modifications of cells in culture
uptake of new genetic material

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