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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

15 - 20 mmol/litre
2-7 mmol/litre
1-2 mmol/litre
7-15 mmol/litre

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

high glutamine concentration is required
high glucose concentration is required
low glutamine concentration is required
low glucose concentration is required

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Creatine phosphate
ATP
Glucose
Lactic acid

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Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

release of genetic information
phenotypic modifications of cells in culture
both (a) and (b)
uptake of new genetic material

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

increase in pH
no loss of cell viability
no change in pH
reduction in the pH of culture hence loss of cell viability

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by controlled addition of glutamine at low level
by removal of ammonia or ammonium from culture medium
All of these
by substituting glutamine by glutamate

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