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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

2-7 mmol/litre
7-15 mmol/litre
1-2 mmol/litre
15 - 20 mmol/litre

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

no change in pH
increase in pH
reduction in the pH of culture hence loss of cell viability
no loss of cell viability

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Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

1-10%
10-15%
15-20%
2-5%

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

metabolism of nutrients in culture media at a rate greater than that required for consumption
All of these
DNA degradation
lipid peroxidation

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

increase in specific lactate production from glucose
incomplete glutamine oxidation
both (a) and (b)
accumulation of ammonia

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

75-105 mmol/litre
55 - 75 mmol/litre
5.5 - 55 mmol/litre
105-150 mmol/litre

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