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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

2-7 mmol/litre
7-15 mmol/litre
15 - 20 mmol/litre
1-2 mmol/litre

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

anaerobic pathway
oxidative pathway
both (a) and (b)
none of these

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

increase in pH
reduction in the pH of culture hence loss of cell viability
no loss of cell viability
no change in pH

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

incomplete glutamine oxidation
both (a) and (b)
increase in specific lactate production from glucose
accumulation of ammonia

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

both (a) and (b)
semi-solid medium
liquid medium
solid medium

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Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

1-10%
2-5%
10-15%
15-20%

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