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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

7-15 mmol/litre
2-7 mmol/litre
15 - 20 mmol/litre
1-2 mmol/litre

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

0.05-5 pmol of O2/cell/h
5 - 10 pmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
0.05-5 nmol of O2/cell/h

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

5.5 - 55 mmol/litre
55 - 75 mmol/litre
75-105 mmol/litre
105-150 mmol/litre

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

DNA degradation
metabolism of nutrients in culture media at a rate greater than that required for consumption
lipid peroxidation
All of these

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Animal Cell Culture and Regulation
Excess CO2 suppress cell growth and productivity by

both (a) and (b)
inhibiting respiration
altering pH of the medium
altering intracellular pH by diffusing across cell membrane

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Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

All of these
Growth factors
Glucose and Glutamine
Cytokines

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