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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

15 - 20 mmol/litre
1-2 mmol/litre
2-7 mmol/litre
7-15 mmol/litre

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

unstable
anchorage independent
stable
anchorage dependent

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Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

All of these
Cytokines
Growth factors
Glucose and Glutamine

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Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

Lactate only
CO2 and NH3
CO2 and lactate
Lactate and ammonium

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

established cell lines
propagated cell lines
primary cell lines
secondary cell lines

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

ATP
Creatine phosphate
Lactic acid
Glucose

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