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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

7-15 mmol/litre
15 - 20 mmol/litre
1-2 mmol/litre
2-7 mmol/litre

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

It participates in the de novo synthesis of purines and pyrimidines
For monitoring pH of the culture
Helps in the cells respiration
All of these

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

They act as growth inhibitors
Have no effect on cells
They act as growth promoters
Lactate helps in the growth while ammonium inhibits the growth

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

presence of CO2
addition of bases
presence of bicarbonate buffer
none of these

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

low glutamine concentration is required
high glucose concentration is required
high glutamine concentration is required
low glucose concentration is required

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Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

phenotypic modifications of cells in culture
release of genetic information
uptake of new genetic material
both (a) and (b)

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