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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

1-2 mmol/litre
7-15 mmol/litre
2-7 mmol/litre
15 - 20 mmol/litre

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Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Adherent primary culture
Slide culture
Roller test tube culture
Carrel flask culture

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Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it doesn't produce solid clots
it is too opaque
it forms a semi solid coagulum
it forms a clear and solid coagulum even after dilution

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

no loss of cell viability
reduction in the pH of culture hence loss of cell viability
increase in pH
no change in pH

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Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

CO2 and lactate
Lactate only
Lactate and ammonium
CO2 and NH3

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

oxidative pathway
both (a) and (b)
anaerobic pathway
none of these

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