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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

1-2 mmol/litre
2-7 mmol/litre
7-15 mmol/litre
15 - 20 mmol/litre

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

increase in pH
no loss of cell viability
reduction in the pH of culture hence loss of cell viability
no change in pH

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

55 - 75 mmol/litre
105-150 mmol/litre
75-105 mmol/litre
5.5 - 55 mmol/litre

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

both (a) and (b)
anaerobic pathway
none of these
oxidative pathway

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

low glucose concentration is required
low glutamine concentration is required
high glucose concentration is required
high glutamine concentration is required

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

none of these
addition of bases
presence of CO2
presence of bicarbonate buffer

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