Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

DNA degradation
lipid peroxidation
All of these
metabolism of nutrients in culture media at a rate greater than that required for consumption

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Animal Cell Culture and Regulation
An established cell line can be called where it has been subcultured at least

40 times at an interval of 3 days between subcultures
70 times at an interval of 1 day between subcultures
70 times at an interval of 3 days between subcultures
50 times at an interval of 3 days between subcultures

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