Animal Cell Culture and Regulation What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells? Lactate helps in the growth while ammonium inhibits the growth They act as growth inhibitors They act as growth promoters Have no effect on cells Lactate helps in the growth while ammonium inhibits the growth They act as growth inhibitors They act as growth promoters Have no effect on cells ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation An established cell line can be called where it has been subcultured at least 50 times at an interval of 3 days between subcultures 70 times at an interval of 1 day between subcultures 70 times at an interval of 3 days between subcultures 40 times at an interval of 3 days between subcultures 50 times at an interval of 3 days between subcultures 70 times at an interval of 1 day between subcultures 70 times at an interval of 3 days between subcultures 40 times at an interval of 3 days between subcultures ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is 360-400 150-300 300-325 280-360 360-400 150-300 300-325 280-360 ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Toxicity due to accumulation of ammonia can be overcome by removal of ammonia or ammonium from culture medium by substituting glutamine by glutamate by controlled addition of glutamine at low level All of these by removal of ammonia or ammonium from culture medium by substituting glutamine by glutamate by controlled addition of glutamine at low level All of these ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Disaggregating of cells can be achieved by enzymatic digestion treating with chelating agents physical disruption All of these enzymatic digestion treating with chelating agents physical disruption All of these ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation To prevent the accumulation of lactate high glucose concentration is required high glutamine concentration is required low glutamine concentration is required low glucose concentration is required high glucose concentration is required high glutamine concentration is required low glutamine concentration is required low glucose concentration is required ANSWER DOWNLOAD EXAMIANS APP
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