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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

Whole embryo cultures
All of these
Organ cultures on plasma clots
Organ cultures on agar

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

All of these
treating with chelating agents
physical disruption
enzymatic digestion

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

none of these
established cell lines
cell transformations
stable primary cell lines

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Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

10-15%
15-20%
1-10%
2-5%

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

280-360
360-400
300-325
150-300

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

All of these
by removal of ammonia or ammonium from culture medium
by controlled addition of glutamine at low level
by substituting glutamine by glutamate

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