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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

Whole embryo cultures
Organ cultures on plasma clots
All of these
Organ cultures on agar

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

75-105 mmol/litre
105-150 mmol/litre
55 - 75 mmol/litre
5.5 - 55 mmol/litre

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Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

release of genetic information
uptake of new genetic material
phenotypic modifications of cells in culture
both (a) and (b)

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

All of these
by substituting glutamine by glutamate
by removal of ammonia or ammonium from culture medium
by controlled addition of glutamine at low level

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

primary cell lines
secondary cell lines
established cell lines
propagated cell lines

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

cell transformations
none of these
stable primary cell lines
established cell lines

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