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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

Organ cultures on plasma clots
Organ cultures on agar
All of these
Whole embryo cultures

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

5.5 - 55 mmol/litre
75-105 mmol/litre
55 - 75 mmol/litre
105-150 mmol/litre

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

enzymatic digestion
treating with chelating agents
All of these
physical disruption

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

no change in pH
increase in pH
no loss of cell viability
reduction in the pH of culture hence loss of cell viability

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

300-325
150-300
360-400
280-360

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

semi-solid medium
both (a) and (b)
solid medium
liquid medium

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