Animal Cell Culture and Regulation
Excess CO2 suppress cell growth and productivity by

altering intracellular pH by diffusing across cell membrane
altering pH of the medium
inhibiting respiration
both (a) and (b)

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Animal Cell Culture and Regulation
An established cell line can be called where it has been subcultured at least

40 times at an interval of 3 days between subcultures
50 times at an interval of 3 days between subcultures
70 times at an interval of 1 day between subcultures
70 times at an interval of 3 days between subcultures

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