Animal Cell Culture and Regulation
Excess CO2 suppress cell growth and productivity by

both (a) and (b)
altering intracellular pH by diffusing across cell membrane
altering pH of the medium
inhibiting respiration

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Animal Cell Culture and Regulation
The major problem associated with the isolation of free cells and cell aggregates from organs is that of

None of these
disintegrating the cells from their supporting matrix
releasing the cells from their supporting matrix
inhibiting the cells from their supporting matrix

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Animal Cell Culture and Regulation
An established cell line can be called where it has been subcultured at least

50 times at an interval of 3 days between subcultures
40 times at an interval of 3 days between subcultures
70 times at an interval of 3 days between subcultures
70 times at an interval of 1 day between subcultures

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