Animal Cell Culture and Regulation Excess CO2 suppress cell growth and productivity by altering pH of the medium both (a) and (b) altering intracellular pH by diffusing across cell membrane inhibiting respiration altering pH of the medium both (a) and (b) altering intracellular pH by diffusing across cell membrane inhibiting respiration ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Accumulation of lactate leads to reduction in the pH of culture hence loss of cell viability no change in pH increase in pH no loss of cell viability reduction in the pH of culture hence loss of cell viability no change in pH increase in pH no loss of cell viability ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively) 5 - 10 nmol of O2 /cell/h 0.05-5 nmol of O2/cell/h 0.05-5 pmol of O2/cell/h 5 - 10 pmol of O2/cell/h 5 - 10 nmol of O2 /cell/h 0.05-5 nmol of O2/cell/h 0.05-5 pmol of O2/cell/h 5 - 10 pmol of O2/cell/h ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of accumulation of ammonia incomplete glutamine oxidation increase in specific lactate production from glucose both (a) and (b) accumulation of ammonia incomplete glutamine oxidation increase in specific lactate production from glucose both (a) and (b) ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation What is the concentration of CO2 required for culturing animal cells? 10-15% 15-20% 1-10% 2-5% 10-15% 15-20% 1-10% 2-5% ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation An established cell line can be called where it has been subcultured at least 50 times at an interval of 3 days between subcultures 40 times at an interval of 3 days between subcultures 70 times at an interval of 3 days between subcultures 70 times at an interval of 1 day between subcultures 50 times at an interval of 3 days between subcultures 40 times at an interval of 3 days between subcultures 70 times at an interval of 3 days between subcultures 70 times at an interval of 1 day between subcultures ANSWER DOWNLOAD EXAMIANS APP
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