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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

none of these
presence of CO2
addition of bases
presence of bicarbonate buffer

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

oxidative pathway
none of these
both (a) and (b)
anaerobic pathway

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

All of these
lipid peroxidation
metabolism of nutrients in culture media at a rate greater than that required for consumption
DNA degradation

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

Have no effect on cells
They act as growth inhibitors
They act as growth promoters
Lactate helps in the growth while ammonium inhibits the growth

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

high glutamine concentration is required
low glucose concentration is required
low glutamine concentration is required
high glucose concentration is required

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

stable primary cell lines
established cell lines
cell transformations
none of these

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