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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

presence of bicarbonate buffer
none of these
addition of bases
presence of CO2

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

All of these
It participates in the de novo synthesis of purines and pyrimidines
Helps in the cells respiration
For monitoring pH of the culture

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Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

1-10%
10-15%
15-20%
2-5%

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

15 - 20 mmol/litre
2-7 mmol/litre
7-15 mmol/litre
1-2 mmol/litre

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

0.05-5 pmol of O2/cell/h
5 - 10 pmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
0.05-5 nmol of O2/cell/h

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Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it forms a semi solid coagulum
it forms a clear and solid coagulum even after dilution
it doesn't produce solid clots
it is too opaque

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