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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

addition of bases
presence of CO2
none of these
presence of bicarbonate buffer

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

280-360
360-400
150-300
300-325

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

established cell lines
cell transformations
stable primary cell lines
none of these

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

liquid medium
solid medium
semi-solid medium
both (a) and (b)

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

55 - 75 mmol/litre
5.5 - 55 mmol/litre
75-105 mmol/litre
105-150 mmol/litre

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

both (a) and (b)
none of these
anaerobic pathway
oxidative pathway

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