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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

addition of bases
none of these
presence of bicarbonate buffer
presence of CO2

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Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Carrel flask culture
Adherent primary culture
Roller test tube culture
Slide culture

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Animal Cell Culture and Regulation
Excess CO2 suppress cell growth and productivity by

altering pH of the medium
inhibiting respiration
both (a) and (b)
altering intracellular pH by diffusing across cell membrane

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

stable
unstable
anchorage dependent
anchorage independent

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

increase in specific lactate production from glucose
accumulation of ammonia
incomplete glutamine oxidation
both (a) and (b)

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

solid medium
semi-solid medium
liquid medium
both (a) and (b)

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