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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

presence of bicarbonate buffer
presence of CO2
addition of bases
none of these

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

propagated cell lines
established cell lines
primary cell lines
secondary cell lines

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

no loss of cell viability
increase in pH
reduction in the pH of culture hence loss of cell viability
no change in pH

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

105-150 mmol/litre
5.5 - 55 mmol/litre
75-105 mmol/litre
55 - 75 mmol/litre

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Lactic acid
Glucose
Creatine phosphate
ATP

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by controlled addition of glutamine at low level
by removal of ammonia or ammonium from culture medium
All of these
by substituting glutamine by glutamate

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