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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

presence of CO2
none of these
addition of bases
presence of bicarbonate buffer

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

stable
anchorage dependent
unstable
anchorage independent

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

primary cell lines
propagated cell lines
secondary cell lines
established cell lines

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Animal Cell Culture and Regulation
Excess CO2 suppress cell growth and productivity by

altering pH of the medium
both (a) and (b)
inhibiting respiration
altering intracellular pH by diffusing across cell membrane

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

physical disruption
treating with chelating agents
All of these
enzymatic digestion

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

2-7 mmol/litre
7-15 mmol/litre
1-2 mmol/litre
15 - 20 mmol/litre

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