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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

presence of CO2
presence of bicarbonate buffer
none of these
addition of bases

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Animal Cell Culture and Regulation
Excess CO2 suppress cell growth and productivity by

altering pH of the medium
both (a) and (b)
inhibiting respiration
altering intracellular pH by diffusing across cell membrane

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

All of these
physical disruption
enzymatic digestion
treating with chelating agents

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

cell transformations
none of these
stable primary cell lines
established cell lines

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

360-400
280-360
300-325
150-300

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

liquid medium
semi-solid medium
solid medium
both (a) and (b)

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