Animal Cell Culture and Regulation
Accumulation of lactate leads to

no loss of cell viability
reduction in the pH of culture hence loss of cell viability
no change in pH
increase in pH

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Animal Cell Culture and Regulation
During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture?

Glycolysis is being inhibited
The cells do not have enough oxygen
Ethyl alcohol is being produced in excess
The cells have too much oxygen

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Animal Cell Culture and Regulation
An established cell line can be called where it has been subcultured at least

70 times at an interval of 3 days between subcultures
70 times at an interval of 1 day between subcultures
50 times at an interval of 3 days between subcultures
40 times at an interval of 3 days between subcultures

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