Animal Cell Culture and Regulation
Accumulation of lactate leads to

no loss of cell viability
increase in pH
reduction in the pH of culture hence loss of cell viability
no change in pH

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Animal Cell Culture and Regulation
An established cell line can be called where it has been subcultured at least

70 times at an interval of 1 day between subcultures
70 times at an interval of 3 days between subcultures
40 times at an interval of 3 days between subcultures
50 times at an interval of 3 days between subcultures

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