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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Slide culture
Roller test tube culture
Carrel flask culture
Adherent primary culture

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

150-300
300-325
360-400
280-360

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

high glutamine concentration is required
high glucose concentration is required
low glutamine concentration is required
low glucose concentration is required

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

1-2 mmol/litre
15 - 20 mmol/litre
2-7 mmol/litre
7-15 mmol/litre

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

oxidative pathway
none of these
anaerobic pathway
both (a) and (b)

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

DNA degradation
All of these
metabolism of nutrients in culture media at a rate greater than that required for consumption
lipid peroxidation

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