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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Carrel flask culture
Roller test tube culture
Adherent primary culture
Slide culture

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

increase in pH
no loss of cell viability
no change in pH
reduction in the pH of culture hence loss of cell viability

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

liquid medium
solid medium
semi-solid medium
both (a) and (b)

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Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it forms a clear and solid coagulum even after dilution
it forms a semi solid coagulum
it doesn't produce solid clots
it is too opaque

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

All of these
metabolism of nutrients in culture media at a rate greater than that required for consumption
lipid peroxidation
DNA degradation

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Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

12 amino acids
6 amino acids
10 amino acids
8 amino acids

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