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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Roller test tube culture
Slide culture
Carrel flask culture
Adherent primary culture

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Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

Organ cultures on agar
Organ cultures on plasma clots
All of these
Whole embryo cultures

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Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

10-15%
15-20%
2-5%
1-10%

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by removal of ammonia or ammonium from culture medium
by substituting glutamine by glutamate
by controlled addition of glutamine at low level
All of these

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

DNA degradation
All of these
lipid peroxidation
metabolism of nutrients in culture media at a rate greater than that required for consumption

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

0.05-5 pmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
5 - 10 pmol of O2/cell/h
0.05-5 nmol of O2/cell/h

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