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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Slide culture
Carrel flask culture
Adherent primary culture
Roller test tube culture

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

5 - 10 nmol of O2 /cell/h
5 - 10 pmol of O2/cell/h
0.05-5 pmol of O2/cell/h
0.05-5 nmol of O2/cell/h

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Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Glucose and Glutamine
All of these
Growth factors
Cytokines

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

105-150 mmol/litre
55 - 75 mmol/litre
75-105 mmol/litre
5.5 - 55 mmol/litre

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

They act as growth promoters
They act as growth inhibitors
Have no effect on cells
Lactate helps in the growth while ammonium inhibits the growth

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

enzymatic digestion
physical disruption
All of these
treating with chelating agents

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