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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Slide culture
Carrel flask culture
Roller test tube culture
Adherent primary culture

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

280-360
360-400
300-325
150-300

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

0.05-5 nmol of O2/cell/h
0.05-5 pmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
5 - 10 pmol of O2/cell/h

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

primary cell lines
propagated cell lines
secondary cell lines
established cell lines

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

15 - 20 mmol/litre
7-15 mmol/litre
2-7 mmol/litre
1-2 mmol/litre

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

5.5 - 55 mmol/litre
105-150 mmol/litre
55 - 75 mmol/litre
75-105 mmol/litre

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