Animal Cell Culture and Regulation When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of both (a) and (b) accumulation of ammonia increase in specific lactate production from glucose incomplete glutamine oxidation both (a) and (b) accumulation of ammonia increase in specific lactate production from glucose incomplete glutamine oxidation ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively) 5 - 10 nmol of O2 /cell/h 5 - 10 pmol of O2/cell/h 0.05-5 nmol of O2/cell/h 0.05-5 pmol of O2/cell/h 5 - 10 nmol of O2 /cell/h 5 - 10 pmol of O2/cell/h 0.05-5 nmol of O2/cell/h 0.05-5 pmol of O2/cell/h ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation An established cell line can be called where it has been subcultured at least 50 times at an interval of 3 days between subcultures 70 times at an interval of 1 day between subcultures 70 times at an interval of 3 days between subcultures 40 times at an interval of 3 days between subcultures 50 times at an interval of 3 days between subcultures 70 times at an interval of 1 day between subcultures 70 times at an interval of 3 days between subcultures 40 times at an interval of 3 days between subcultures ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Excess CO2 suppress cell growth and productivity by both (a) and (b) altering intracellular pH by diffusing across cell membrane altering pH of the medium inhibiting respiration both (a) and (b) altering intracellular pH by diffusing across cell membrane altering pH of the medium inhibiting respiration ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation pH of culture medium is initially controlled by none of these addition of bases presence of CO2 presence of bicarbonate buffer none of these addition of bases presence of CO2 presence of bicarbonate buffer ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via both (a) and (b) anaerobic pathway none of these oxidative pathway both (a) and (b) anaerobic pathway none of these oxidative pathway ANSWER DOWNLOAD EXAMIANS APP
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