Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

both (a) and (b)
accumulation of ammonia
increase in specific lactate production from glucose
incomplete glutamine oxidation

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Animal Cell Culture and Regulation
An established cell line can be called where it has been subcultured at least

50 times at an interval of 3 days between subcultures
70 times at an interval of 1 day between subcultures
70 times at an interval of 3 days between subcultures
40 times at an interval of 3 days between subcultures

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