Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

both (a) and (b)
incomplete glutamine oxidation
accumulation of ammonia
increase in specific lactate production from glucose

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Animal Cell Culture and Regulation
An established cell line can be called where it has been subcultured at least

40 times at an interval of 3 days between subcultures
70 times at an interval of 3 days between subcultures
50 times at an interval of 3 days between subcultures
70 times at an interval of 1 day between subcultures

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