Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

incomplete glutamine oxidation
accumulation of ammonia
increase in specific lactate production from glucose
both (a) and (b)

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Animal Cell Culture and Regulation
The major problem associated with the isolation of free cells and cell aggregates from organs is that of

releasing the cells from their supporting matrix
inhibiting the cells from their supporting matrix
disintegrating the cells from their supporting matrix
None of these

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