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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

All of these
Glucose and Glutamine
Growth factors
Cytokines

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

5 - 10 pmol of O2/cell/h
0.05-5 nmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
0.05-5 pmol of O2/cell/h

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

none of these
stable primary cell lines
established cell lines
cell transformations

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Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

12 amino acids
6 amino acids
10 amino acids
8 amino acids

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

280-360
300-325
150-300
360-400

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Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Roller test tube culture
Slide culture
Adherent primary culture
Carrel flask culture

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