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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Glucose and Glutamine
Cytokines
All of these
Growth factors

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Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

release of genetic information
uptake of new genetic material
both (a) and (b)
phenotypic modifications of cells in culture

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

0.05-5 nmol of O2/cell/h
0.05-5 pmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
5 - 10 pmol of O2/cell/h

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Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

10 amino acids
12 amino acids
6 amino acids
8 amino acids

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

none of these
addition of bases
presence of CO2
presence of bicarbonate buffer

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

5.5 - 55 mmol/litre
55 - 75 mmol/litre
105-150 mmol/litre
75-105 mmol/litre

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