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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Growth factors
All of these
Cytokines
Glucose and Glutamine

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

incomplete glutamine oxidation
both (a) and (b)
increase in specific lactate production from glucose
accumulation of ammonia

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

presence of CO2
addition of bases
presence of bicarbonate buffer
none of these

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

unstable
anchorage dependent
anchorage independent
stable

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Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

10-15%
2-5%
1-10%
15-20%

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Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

CO2 and NH3
CO2 and lactate
Lactate and ammonium
Lactate only

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