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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

All of these
Growth factors
Glucose and Glutamine
Cytokines

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

enzymatic digestion
All of these
treating with chelating agents
physical disruption

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Animal Cell Culture and Regulation
Excess CO2 suppress cell growth and productivity by

altering intracellular pH by diffusing across cell membrane
both (a) and (b)
inhibiting respiration
altering pH of the medium

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

DNA degradation
metabolism of nutrients in culture media at a rate greater than that required for consumption
All of these
lipid peroxidation

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

0.05-5 nmol of O2/cell/h
0.05-5 pmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
5 - 10 pmol of O2/cell/h

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Animal Cell Culture and Regulation
Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping?

Turner's syndrome
Down's syndrome
all of these
Klinefelter's syndrome

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