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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Glucose and Glutamine
Growth factors
Cytokines
All of these

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Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

CO2 and lactate
Lactate and ammonium
Lactate only
CO2 and NH3

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

All of these
by controlled addition of glutamine at low level
by substituting glutamine by glutamate
by removal of ammonia or ammonium from culture medium

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

high glutamine concentration is required
high glucose concentration is required
low glutamine concentration is required
low glucose concentration is required

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

anaerobic pathway
none of these
both (a) and (b)
oxidative pathway

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

primary cell lines
secondary cell lines
propagated cell lines
established cell lines

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