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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Growth factors
All of these
Cytokines
Glucose and Glutamine

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

established cell lines
cell transformations
none of these
stable primary cell lines

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

All of these
For monitoring pH of the culture
Helps in the cells respiration
It participates in the de novo synthesis of purines and pyrimidines

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

0.05-5 nmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
5 - 10 pmol of O2/cell/h
0.05-5 pmol of O2/cell/h

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Animal Cell Culture and Regulation
Excess CO2 suppress cell growth and productivity by

altering intracellular pH by diffusing across cell membrane
altering pH of the medium
both (a) and (b)
inhibiting respiration

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

established cell lines
primary cell lines
propagated cell lines
secondary cell lines

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