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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Cytokines
Growth factors
Glucose and Glutamine
All of these

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

anchorage independent
anchorage dependent
unstable
stable

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

metabolism of nutrients in culture media at a rate greater than that required for consumption
DNA degradation
lipid peroxidation
All of these

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Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

CO2 and NH3
CO2 and lactate
Lactate only
Lactate and ammonium

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

105-150 mmol/litre
5.5 - 55 mmol/litre
55 - 75 mmol/litre
75-105 mmol/litre

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

It participates in the de novo synthesis of purines and pyrimidines
All of these
For monitoring pH of the culture
Helps in the cells respiration

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