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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

All of these
Growth factors
Glucose and Glutamine
Cytokines

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

All of these
treating with chelating agents
physical disruption
enzymatic digestion

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Creatine phosphate
ATP
Lactic acid
Glucose

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

2-7 mmol/litre
7-15 mmol/litre
15 - 20 mmol/litre
1-2 mmol/litre

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

no change in pH
reduction in the pH of culture hence loss of cell viability
no loss of cell viability
increase in pH

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

both (a) and (b)
none of these
anaerobic pathway
oxidative pathway

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