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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Growth factors
Cytokines
All of these
Glucose and Glutamine

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Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

10 amino acids
12 amino acids
6 amino acids
8 amino acids

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

1-2 mmol/litre
2-7 mmol/litre
7-15 mmol/litre
15 - 20 mmol/litre

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

360-400
300-325
280-360
150-300

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

Lactate helps in the growth while ammonium inhibits the growth
They act as growth inhibitors
Have no effect on cells
They act as growth promoters

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

It participates in the de novo synthesis of purines and pyrimidines
For monitoring pH of the culture
All of these
Helps in the cells respiration

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