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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Glucose and Glutamine
All of these
Cytokines
Growth factors

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Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

both (a) and (b)
phenotypic modifications of cells in culture
uptake of new genetic material
release of genetic information

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

1-2 mmol/litre
7-15 mmol/litre
15 - 20 mmol/litre
2-7 mmol/litre

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

anchorage independent
unstable
stable
anchorage dependent

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Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it doesn't produce solid clots
it forms a semi solid coagulum
it forms a clear and solid coagulum even after dilution
it is too opaque

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

both (a) and (b)
incomplete glutamine oxidation
increase in specific lactate production from glucose
accumulation of ammonia

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