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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

All of these
Glucose and Glutamine
Cytokines
Growth factors

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Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

Lactate only
Lactate and ammonium
CO2 and NH3
CO2 and lactate

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by removal of ammonia or ammonium from culture medium
by controlled addition of glutamine at low level
by substituting glutamine by glutamate
All of these

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

7-15 mmol/litre
2-7 mmol/litre
15 - 20 mmol/litre
1-2 mmol/litre

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Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

Organ cultures on agar
All of these
Whole embryo cultures
Organ cultures on plasma clots

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

105-150 mmol/litre
75-105 mmol/litre
55 - 75 mmol/litre
5.5 - 55 mmol/litre

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