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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Glucose and Glutamine
All of these
Cytokines
Growth factors

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

All of these
treating with chelating agents
enzymatic digestion
physical disruption

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Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

uptake of new genetic material
release of genetic information
phenotypic modifications of cells in culture
both (a) and (b)

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

both (a) and (b)
increase in specific lactate production from glucose
incomplete glutamine oxidation
accumulation of ammonia

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

none of these
stable primary cell lines
established cell lines
cell transformations

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by controlled addition of glutamine at low level
by removal of ammonia or ammonium from culture medium
All of these
by substituting glutamine by glutamate

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