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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Cytokines
Growth factors
Glucose and Glutamine
All of these

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

solid medium
liquid medium
semi-solid medium
both (a) and (b)

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

none of these
oxidative pathway
both (a) and (b)
anaerobic pathway

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

both (a) and (b)
increase in specific lactate production from glucose
incomplete glutamine oxidation
accumulation of ammonia

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

stable
anchorage independent
anchorage dependent
unstable

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

presence of bicarbonate buffer
addition of bases
presence of CO2
none of these

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