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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Cytokines
Glucose and Glutamine
All of these
Growth factors

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

incomplete glutamine oxidation
increase in specific lactate production from glucose
both (a) and (b)
accumulation of ammonia

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

cell transformations
established cell lines
none of these
stable primary cell lines

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Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Adherent primary culture
Roller test tube culture
Slide culture
Carrel flask culture

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

15 - 20 mmol/litre
1-2 mmol/litre
7-15 mmol/litre
2-7 mmol/litre

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Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it forms a clear and solid coagulum even after dilution
it is too opaque
it doesn't produce solid clots
it forms a semi solid coagulum

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