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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

All of these
Cytokines
Growth factors
Glucose and Glutamine

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

semi-solid medium
liquid medium
both (a) and (b)
solid medium

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

stable primary cell lines
none of these
established cell lines
cell transformations

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

0.05-5 nmol of O2/cell/h
5 - 10 pmol of O2/cell/h
0.05-5 pmol of O2/cell/h
5 - 10 nmol of O2 /cell/h

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Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

CO2 and NH3
CO2 and lactate
Lactate only
Lactate and ammonium

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

no change in pH
reduction in the pH of culture hence loss of cell viability
increase in pH
no loss of cell viability

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