Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

phenotypic modifications of cells in culture
uptake of new genetic material
both (a) and (b)
release of genetic information

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Animal Cell Culture and Regulation
An established cell line can be called where it has been subcultured at least

70 times at an interval of 1 day between subcultures
50 times at an interval of 3 days between subcultures
40 times at an interval of 3 days between subcultures
70 times at an interval of 3 days between subcultures

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