Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

For monitoring pH of the culture
It participates in the de novo synthesis of purines and pyrimidines
Helps in the cells respiration
All of these

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Animal Cell Culture and Regulation
An established cell line can be called where it has been subcultured at least

70 times at an interval of 3 days between subcultures
70 times at an interval of 1 day between subcultures
50 times at an interval of 3 days between subcultures
40 times at an interval of 3 days between subcultures

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