Animal Cell Culture and Regulation
An established cell line can be called where it has been subcultured at least

40 times at an interval of 3 days between subcultures
70 times at an interval of 1 day between subcultures
50 times at an interval of 3 days between subcultures
70 times at an interval of 3 days between subcultures

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Animal Cell Culture and Regulation
The major problem associated with the isolation of free cells and cell aggregates from organs is that of

releasing the cells from their supporting matrix
disintegrating the cells from their supporting matrix
None of these
inhibiting the cells from their supporting matrix

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