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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

both (a) and (b)
liquid medium
solid medium
semi-solid medium

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by controlled addition of glutamine at low level
All of these
by removal of ammonia or ammonium from culture medium
by substituting glutamine by glutamate

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Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

Whole embryo cultures
Organ cultures on agar
Organ cultures on plasma clots
All of these

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

enzymatic digestion
physical disruption
All of these
treating with chelating agents

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

incomplete glutamine oxidation
accumulation of ammonia
both (a) and (b)
increase in specific lactate production from glucose

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

280-360
360-400
150-300
300-325

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