Animal Cell Culture and Regulation Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is 360-400 150-300 300-325 280-360 360-400 150-300 300-325 280-360 ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation An established cell line can be called where it has been subcultured at least 50 times at an interval of 3 days between subcultures 70 times at an interval of 3 days between subcultures 40 times at an interval of 3 days between subcultures 70 times at an interval of 1 day between subcultures 50 times at an interval of 3 days between subcultures 70 times at an interval of 3 days between subcultures 40 times at an interval of 3 days between subcultures 70 times at an interval of 1 day between subcultures ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation pH of culture medium is initially controlled by presence of CO2 addition of bases presence of bicarbonate buffer none of these presence of CO2 addition of bases presence of bicarbonate buffer none of these ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation How does CO2 help in the cell metabolism during cell culture? It participates in the de novo synthesis of purines and pyrimidines All of these For monitoring pH of the culture Helps in the cells respiration It participates in the de novo synthesis of purines and pyrimidines All of these For monitoring pH of the culture Helps in the cells respiration ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Excess CO2 suppress cell growth and productivity by both (a) and (b) altering intracellular pH by diffusing across cell membrane altering pH of the medium inhibiting respiration both (a) and (b) altering intracellular pH by diffusing across cell membrane altering pH of the medium inhibiting respiration ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Toxicity due to accumulation of ammonia can be overcome by substituting glutamine by glutamate by removal of ammonia or ammonium from culture medium by controlled addition of glutamine at low level All of these by substituting glutamine by glutamate by removal of ammonia or ammonium from culture medium by controlled addition of glutamine at low level All of these ANSWER DOWNLOAD EXAMIANS APP
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