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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

150-300
280-360
360-400
300-325

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

treating with chelating agents
All of these
physical disruption
enzymatic digestion

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

75-105 mmol/litre
105-150 mmol/litre
55 - 75 mmol/litre
5.5 - 55 mmol/litre

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

accumulation of ammonia
both (a) and (b)
incomplete glutamine oxidation
increase in specific lactate production from glucose

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Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

Organ cultures on plasma clots
All of these
Whole embryo cultures
Organ cultures on agar

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

liquid medium
solid medium
both (a) and (b)
semi-solid medium

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