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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

300-325
360-400
150-300
280-360

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

All of these
lipid peroxidation
DNA degradation
metabolism of nutrients in culture media at a rate greater than that required for consumption

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

physical disruption
enzymatic digestion
All of these
treating with chelating agents

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Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

Lactate and ammonium
CO2 and NH3
Lactate only
CO2 and lactate

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

cell transformations
none of these
established cell lines
stable primary cell lines

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

5 - 10 pmol of O2/cell/h
0.05-5 pmol of O2/cell/h
0.05-5 nmol of O2/cell/h
5 - 10 nmol of O2 /cell/h

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