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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

280-360
360-400
300-325
150-300

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

cell transformations
none of these
established cell lines
stable primary cell lines

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

high glucose concentration is required
high glutamine concentration is required
low glucose concentration is required
low glutamine concentration is required

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

both (a) and (b)
incomplete glutamine oxidation
accumulation of ammonia
increase in specific lactate production from glucose

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

enzymatic digestion
treating with chelating agents
All of these
physical disruption

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Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

release of genetic information
uptake of new genetic material
phenotypic modifications of cells in culture
both (a) and (b)

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