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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

360-400
300-325
150-300
280-360

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

105-150 mmol/litre
75-105 mmol/litre
55 - 75 mmol/litre
5.5 - 55 mmol/litre

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

none of these
cell transformations
stable primary cell lines
established cell lines

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

addition of bases
none of these
presence of CO2
presence of bicarbonate buffer

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

They act as growth promoters
Lactate helps in the growth while ammonium inhibits the growth
Have no effect on cells
They act as growth inhibitors

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

All of these
DNA degradation
metabolism of nutrients in culture media at a rate greater than that required for consumption
lipid peroxidation

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