Animal Cell Culture and Regulation Toxicity due to accumulation of ammonia can be overcome by controlled addition of glutamine at low level by substituting glutamine by glutamate All of these by removal of ammonia or ammonium from culture medium by controlled addition of glutamine at low level by substituting glutamine by glutamate All of these by removal of ammonia or ammonium from culture medium ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called secondary cell lines primary cell lines established cell lines propagated cell lines secondary cell lines primary cell lines established cell lines propagated cell lines ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of both (a) and (b) increase in specific lactate production from glucose accumulation of ammonia incomplete glutamine oxidation both (a) and (b) increase in specific lactate production from glucose accumulation of ammonia incomplete glutamine oxidation ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation What is the concentration of CO2 required for culturing animal cells? 2-5% 1-10% 10-15% 15-20% 2-5% 1-10% 10-15% 15-20% ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation To prevent the accumulation of lactate low glucose concentration is required high glucose concentration is required high glutamine concentration is required low glutamine concentration is required low glucose concentration is required high glucose concentration is required high glutamine concentration is required low glutamine concentration is required ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation An established cell line can be called where it has been subcultured at least 70 times at an interval of 1 day between subcultures 40 times at an interval of 3 days between subcultures 50 times at an interval of 3 days between subcultures 70 times at an interval of 3 days between subcultures 70 times at an interval of 1 day between subcultures 40 times at an interval of 3 days between subcultures 50 times at an interval of 3 days between subcultures 70 times at an interval of 3 days between subcultures ANSWER DOWNLOAD EXAMIANS APP
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