Animal Cell Culture and Regulation Toxicity due to accumulation of ammonia can be overcome by controlled addition of glutamine at low level by removal of ammonia or ammonium from culture medium All of these by substituting glutamine by glutamate by controlled addition of glutamine at low level by removal of ammonia or ammonium from culture medium All of these by substituting glutamine by glutamate ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Which of the following is the technique used for the embryo culture? All of these Organ cultures on plasma clots Organ cultures on agar Whole embryo cultures All of these Organ cultures on plasma clots Organ cultures on agar Whole embryo cultures ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation How does CO2 help in the cell metabolism during cell culture? For monitoring pH of the culture Helps in the cells respiration It participates in the de novo synthesis of purines and pyrimidines All of these For monitoring pH of the culture Helps in the cells respiration It participates in the de novo synthesis of purines and pyrimidines All of these ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of both (a) and (b) accumulation of ammonia incomplete glutamine oxidation increase in specific lactate production from glucose both (a) and (b) accumulation of ammonia incomplete glutamine oxidation increase in specific lactate production from glucose ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively) 0.05-5 pmol of O2/cell/h 0.05-5 nmol of O2/cell/h 5 - 10 nmol of O2 /cell/h 5 - 10 pmol of O2/cell/h 0.05-5 pmol of O2/cell/h 0.05-5 nmol of O2/cell/h 5 - 10 nmol of O2 /cell/h 5 - 10 pmol of O2/cell/h ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is 360-400 150-300 300-325 280-360 360-400 150-300 300-325 280-360 ANSWER DOWNLOAD EXAMIANS APP