Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by substituting glutamine by glutamate
by removal of ammonia or ammonium from culture medium
by controlled addition of glutamine at low level
All of these

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Animal Cell Culture and Regulation
During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture?

Ethyl alcohol is being produced in excess
Glycolysis is being inhibited
The cells do not have enough oxygen
The cells have too much oxygen

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