Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by controlled addition of glutamine at low level
All of these
by removal of ammonia or ammonium from culture medium
by substituting glutamine by glutamate

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Animal Cell Culture and Regulation
The major problem associated with the isolation of free cells and cell aggregates from organs is that of

releasing the cells from their supporting matrix
inhibiting the cells from their supporting matrix
disintegrating the cells from their supporting matrix
None of these

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