Animal Cell Culture and Regulation
To prevent the accumulation of lactate

low glucose concentration is required
high glucose concentration is required
low glutamine concentration is required
high glutamine concentration is required

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Animal Cell Culture and Regulation
An established cell line can be called where it has been subcultured at least

70 times at an interval of 1 day between subcultures
40 times at an interval of 3 days between subcultures
70 times at an interval of 3 days between subcultures
50 times at an interval of 3 days between subcultures

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