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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

75-105 mmol/litre
55 - 75 mmol/litre
105-150 mmol/litre
5.5 - 55 mmol/litre

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

accumulation of ammonia
both (a) and (b)
incomplete glutamine oxidation
increase in specific lactate production from glucose

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

0.05-5 pmol of O2/cell/h
5 - 10 pmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
0.05-5 nmol of O2/cell/h

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Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

1-10%
2-5%
15-20%
10-15%

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

280-360
300-325
150-300
360-400

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

unstable
stable
anchorage dependent
anchorage independent

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