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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

75-105 mmol/litre
105-150 mmol/litre
55 - 75 mmol/litre
5.5 - 55 mmol/litre

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

0.05-5 nmol of O2/cell/h
5 - 10 pmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
0.05-5 pmol of O2/cell/h

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Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

12 amino acids
10 amino acids
6 amino acids
8 amino acids

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

presence of bicarbonate buffer
addition of bases
none of these
presence of CO2

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Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

Lactate and ammonium
CO2 and NH3
Lactate only
CO2 and lactate

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

none of these
cell transformations
stable primary cell lines
established cell lines

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