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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

105-150 mmol/litre
55 - 75 mmol/litre
75-105 mmol/litre
5.5 - 55 mmol/litre

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Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Roller test tube culture
Slide culture
Carrel flask culture
Adherent primary culture

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

solid medium
semi-solid medium
both (a) and (b)
liquid medium

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Animal Cell Culture and Regulation
Excess CO2 suppress cell growth and productivity by

altering intracellular pH by diffusing across cell membrane
both (a) and (b)
altering pH of the medium
inhibiting respiration

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

Have no effect on cells
Lactate helps in the growth while ammonium inhibits the growth
They act as growth promoters
They act as growth inhibitors

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

All of these
treating with chelating agents
enzymatic digestion
physical disruption

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