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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

5.5 - 55 mmol/litre
75-105 mmol/litre
105-150 mmol/litre
55 - 75 mmol/litre

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

They act as growth inhibitors
Lactate helps in the growth while ammonium inhibits the growth
They act as growth promoters
Have no effect on cells

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

unstable
anchorage independent
stable
anchorage dependent

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

oxidative pathway
anaerobic pathway
both (a) and (b)
none of these

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Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

All of these
Whole embryo cultures
Organ cultures on agar
Organ cultures on plasma clots

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

low glutamine concentration is required
high glutamine concentration is required
low glucose concentration is required
high glucose concentration is required

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