Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

5 - 10 pmol of O2/cell/h
0.05-5 nmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
0.05-5 pmol of O2/cell/h

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Animal Cell Culture and Regulation
An established cell line can be called where it has been subcultured at least

50 times at an interval of 3 days between subcultures
70 times at an interval of 3 days between subcultures
40 times at an interval of 3 days between subcultures
70 times at an interval of 1 day between subcultures

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Animal Cell Culture and Regulation
The major problem associated with the isolation of free cells and cell aggregates from organs is that of

disintegrating the cells from their supporting matrix
inhibiting the cells from their supporting matrix
None of these
releasing the cells from their supporting matrix

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