Animal Cell Culture and Regulation Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively) 0.05-5 pmol of O2/cell/h 5 - 10 pmol of O2/cell/h 0.05-5 nmol of O2/cell/h 5 - 10 nmol of O2 /cell/h 0.05-5 pmol of O2/cell/h 5 - 10 pmol of O2/cell/h 0.05-5 nmol of O2/cell/h 5 - 10 nmol of O2 /cell/h ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation The human fibroplast is a classical example of cell transformations none of these established cell lines stable primary cell lines cell transformations none of these established cell lines stable primary cell lines ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of accumulation of ammonia both (a) and (b) increase in specific lactate production from glucose incomplete glutamine oxidation accumulation of ammonia both (a) and (b) increase in specific lactate production from glucose incomplete glutamine oxidation ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Toxicity due to accumulation of ammonia can be overcome by controlled addition of glutamine at low level All of these by removal of ammonia or ammonium from culture medium by substituting glutamine by glutamate by controlled addition of glutamine at low level All of these by removal of ammonia or ammonium from culture medium by substituting glutamine by glutamate ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation The major problem associated with the isolation of free cells and cell aggregates from organs is that of None of these inhibiting the cells from their supporting matrix disintegrating the cells from their supporting matrix releasing the cells from their supporting matrix None of these inhibiting the cells from their supporting matrix disintegrating the cells from their supporting matrix releasing the cells from their supporting matrix ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called established cell lines primary cell lines secondary cell lines propagated cell lines established cell lines primary cell lines secondary cell lines propagated cell lines ANSWER DOWNLOAD EXAMIANS APP
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