Animal Cell Culture and Regulation Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively) 5 - 10 nmol of O2 /cell/h 0.05-5 pmol of O2/cell/h 5 - 10 pmol of O2/cell/h 0.05-5 nmol of O2/cell/h 5 - 10 nmol of O2 /cell/h 0.05-5 pmol of O2/cell/h 5 - 10 pmol of O2/cell/h 0.05-5 nmol of O2/cell/h ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Toxicity due to accumulation of ammonia can be overcome All of these by substituting glutamine by glutamate by controlled addition of glutamine at low level by removal of ammonia or ammonium from culture medium All of these by substituting glutamine by glutamate by controlled addition of glutamine at low level by removal of ammonia or ammonium from culture medium ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation An established cell line can be called where it has been subcultured at least 70 times at an interval of 3 days between subcultures 70 times at an interval of 1 day between subcultures 40 times at an interval of 3 days between subcultures 50 times at an interval of 3 days between subcultures 70 times at an interval of 3 days between subcultures 70 times at an interval of 1 day between subcultures 40 times at an interval of 3 days between subcultures 50 times at an interval of 3 days between subcultures ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation What are the main constituents of culture for animal cell growth? Growth factors Cytokines Glucose and Glutamine All of these Growth factors Cytokines Glucose and Glutamine All of these ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells? They act as growth inhibitors Lactate helps in the growth while ammonium inhibits the growth They act as growth promoters Have no effect on cells They act as growth inhibitors Lactate helps in the growth while ammonium inhibits the growth They act as growth promoters Have no effect on cells ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of both (a) and (b) increase in specific lactate production from glucose accumulation of ammonia incomplete glutamine oxidation both (a) and (b) increase in specific lactate production from glucose accumulation of ammonia incomplete glutamine oxidation ANSWER DOWNLOAD EXAMIANS APP
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