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ANIMAL CELL CULTURE AND REGULATION

Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it doesn't produce solid clots
it is too opaque
it forms a semi solid coagulum
it forms a clear and solid coagulum even after dilution

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by controlled addition of glutamine at low level
by substituting glutamine by glutamate
by removal of ammonia or ammonium from culture medium
All of these

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

300-325
280-360
360-400
150-300

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

physical disruption
enzymatic digestion
All of these
treating with chelating agents

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

no change in pH
increase in pH
reduction in the pH of culture hence loss of cell viability
no loss of cell viability

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