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ANIMAL CELL CULTURE AND REGULATION

Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it forms a semi solid coagulum
it forms a clear and solid coagulum even after dilution
it is too opaque
it doesn't produce solid clots

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by removal of ammonia or ammonium from culture medium
All of these
by substituting glutamine by glutamate
by controlled addition of glutamine at low level

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

280-360
300-325
150-300
360-400

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

All of these
physical disruption
treating with chelating agents
enzymatic digestion

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

reduction in the pH of culture hence loss of cell viability
no change in pH
no loss of cell viability
increase in pH

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