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ANIMAL CELL CULTURE AND REGULATION

Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

increase in specific lactate production from glucose
accumulation of ammonia
both (a) and (b)
incomplete glutamine oxidation

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Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

12 amino acids
10 amino acids
8 amino acids
6 amino acids

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Animal Cell Culture and Regulation
Excess CO2 suppress cell growth and productivity by

altering pH of the medium
inhibiting respiration
altering intracellular pH by diffusing across cell membrane
both (a) and (b)

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

They act as growth inhibitors
They act as growth promoters
Lactate helps in the growth while ammonium inhibits the growth
Have no effect on cells

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Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

15-20%
1-10%
10-15%
2-5%

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