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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

2-5%
15-20%
10-15%
1-10%

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

accumulation of ammonia
increase in specific lactate production from glucose
incomplete glutamine oxidation
both (a) and (b)

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

propagated cell lines
secondary cell lines
primary cell lines
established cell lines

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Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

phenotypic modifications of cells in culture
both (a) and (b)
uptake of new genetic material
release of genetic information

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Creatine phosphate
Glucose
ATP
Lactic acid

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

high glutamine concentration is required
low glutamine concentration is required
low glucose concentration is required
high glucose concentration is required

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