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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

2-5%
10-15%
1-10%
15-20%

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by controlled addition of glutamine at low level
All of these
by substituting glutamine by glutamate
by removal of ammonia or ammonium from culture medium

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

secondary cell lines
established cell lines
propagated cell lines
primary cell lines

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Creatine phosphate
Lactic acid
ATP
Glucose

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

low glutamine concentration is required
high glucose concentration is required
low glucose concentration is required
high glutamine concentration is required

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Animal Cell Culture and Regulation
Excess CO2 suppress cell growth and productivity by

altering pH of the medium
altering intracellular pH by diffusing across cell membrane
both (a) and (b)
inhibiting respiration

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