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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

15-20%
10-15%
1-10%
2-5%

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

Lactate helps in the growth while ammonium inhibits the growth
They act as growth inhibitors
They act as growth promoters
Have no effect on cells

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

none of these
both (a) and (b)
oxidative pathway
anaerobic pathway

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Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

All of these
Organ cultures on agar
Whole embryo cultures
Organ cultures on plasma clots

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

accumulation of ammonia
increase in specific lactate production from glucose
incomplete glutamine oxidation
both (a) and (b)

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Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

6 amino acids
8 amino acids
12 amino acids
10 amino acids

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