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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

10-15%
15-20%
2-5%
1-10%

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

DNA degradation
metabolism of nutrients in culture media at a rate greater than that required for consumption
lipid peroxidation
All of these

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Animal Cell Culture and Regulation
Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping?

Klinefelter's syndrome
all of these
Turner's syndrome
Down's syndrome

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

75-105 mmol/litre
105-150 mmol/litre
5.5 - 55 mmol/litre
55 - 75 mmol/litre

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

0.05-5 pmol of O2/cell/h
0.05-5 nmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
5 - 10 pmol of O2/cell/h

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

none of these
oxidative pathway
anaerobic pathway
both (a) and (b)

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