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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

15-20%
2-5%
10-15%
1-10%

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

All of these
DNA degradation
metabolism of nutrients in culture media at a rate greater than that required for consumption
lipid peroxidation

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

unstable
anchorage independent
anchorage dependent
stable

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Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Slide culture
Roller test tube culture
Adherent primary culture
Carrel flask culture

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

oxidative pathway
both (a) and (b)
anaerobic pathway
none of these

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

solid medium
liquid medium
semi-solid medium
both (a) and (b)

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