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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

15-20%
1-10%
10-15%
2-5%

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

7-15 mmol/litre
2-7 mmol/litre
15 - 20 mmol/litre
1-2 mmol/litre

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Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

Whole embryo cultures
Organ cultures on agar
Organ cultures on plasma clots
All of these

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

physical disruption
All of these
treating with chelating agents
enzymatic digestion

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Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

12 amino acids
8 amino acids
10 amino acids
6 amino acids

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Animal Cell Culture and Regulation
Excess CO2 suppress cell growth and productivity by

altering pH of the medium
inhibiting respiration
altering intracellular pH by diffusing across cell membrane
both (a) and (b)

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