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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

10-15%
1-10%
15-20%
2-5%

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

both (a) and (b)
accumulation of ammonia
increase in specific lactate production from glucose
incomplete glutamine oxidation

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Creatine phosphate
ATP
Glucose
Lactic acid

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

5 - 10 nmol of O2 /cell/h
0.05-5 nmol of O2/cell/h
5 - 10 pmol of O2/cell/h
0.05-5 pmol of O2/cell/h

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Animal Cell Culture and Regulation
Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping?

Down's syndrome
Turner's syndrome
Klinefelter's syndrome
all of these

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

no loss of cell viability
reduction in the pH of culture hence loss of cell viability
no change in pH
increase in pH

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