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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

2-5%
10-15%
15-20%
1-10%

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

1-2 mmol/litre
7-15 mmol/litre
15 - 20 mmol/litre
2-7 mmol/litre

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

semi-solid medium
solid medium
liquid medium
both (a) and (b)

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

presence of CO2
addition of bases
none of these
presence of bicarbonate buffer

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

stable
unstable
anchorage dependent
anchorage independent

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

150-300
300-325
280-360
360-400

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