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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

15-20%
1-10%
10-15%
2-5%

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

high glutamine concentration is required
high glucose concentration is required
low glutamine concentration is required
low glucose concentration is required

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

enzymatic digestion
physical disruption
All of these
treating with chelating agents

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Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

8 amino acids
10 amino acids
12 amino acids
6 amino acids

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

propagated cell lines
established cell lines
primary cell lines
secondary cell lines

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by removal of ammonia or ammonium from culture medium
by controlled addition of glutamine at low level
by substituting glutamine by glutamate
All of these

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