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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

10-15%
15-20%
2-5%
1-10%

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

For monitoring pH of the culture
Helps in the cells respiration
All of these
It participates in the de novo synthesis of purines and pyrimidines

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

physical disruption
All of these
treating with chelating agents
enzymatic digestion

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

solid medium
liquid medium
semi-solid medium
both (a) and (b)

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

none of these
both (a) and (b)
oxidative pathway
anaerobic pathway

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

none of these
established cell lines
stable primary cell lines
cell transformations

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