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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

10-15%
2-5%
15-20%
1-10%

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Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

10 amino acids
8 amino acids
12 amino acids
6 amino acids

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by controlled addition of glutamine at low level
by removal of ammonia or ammonium from culture medium
All of these
by substituting glutamine by glutamate

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

All of these
Helps in the cells respiration
It participates in the de novo synthesis of purines and pyrimidines
For monitoring pH of the culture

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

0.05-5 pmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
5 - 10 pmol of O2/cell/h
0.05-5 nmol of O2/cell/h

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

280-360
300-325
360-400
150-300

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