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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

2-5%
1-10%
15-20%
10-15%

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

DNA degradation
All of these
lipid peroxidation
metabolism of nutrients in culture media at a rate greater than that required for consumption

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

It participates in the de novo synthesis of purines and pyrimidines
For monitoring pH of the culture
All of these
Helps in the cells respiration

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Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

CO2 and NH3
Lactate only
Lactate and ammonium
CO2 and lactate

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

stable
anchorage independent
unstable
anchorage dependent

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

360-400
280-360
150-300
300-325

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