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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
The human fibroplast is a classical example of

stable primary cell lines
none of these
cell transformations
established cell lines

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

primary cell lines
established cell lines
secondary cell lines
propagated cell lines

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

55 - 75 mmol/litre
5.5 - 55 mmol/litre
75-105 mmol/litre
105-150 mmol/litre

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Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it is too opaque
it forms a semi solid coagulum
it forms a clear and solid coagulum even after dilution
it doesn't produce solid clots

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

360-400
300-325
150-300
280-360

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

presence of bicarbonate buffer
none of these
addition of bases
presence of CO2

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