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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
The human fibroplast is a classical example of

established cell lines
cell transformations
none of these
stable primary cell lines

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

no change in pH
reduction in the pH of culture hence loss of cell viability
no loss of cell viability
increase in pH

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Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Adherent primary culture
Slide culture
Roller test tube culture
Carrel flask culture

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Lactic acid
Creatine phosphate
ATP
Glucose

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

280-360
300-325
150-300
360-400

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Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

both (a) and (b)
uptake of new genetic material
phenotypic modifications of cells in culture
release of genetic information

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