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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
The human fibroplast is a classical example of

stable primary cell lines
established cell lines
none of these
cell transformations

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

increase in specific lactate production from glucose
accumulation of ammonia
incomplete glutamine oxidation
both (a) and (b)

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

300-325
150-300
360-400
280-360

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

none of these
presence of bicarbonate buffer
addition of bases
presence of CO2

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

increase in pH
no loss of cell viability
reduction in the pH of culture hence loss of cell viability
no change in pH

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

All of these
DNA degradation
metabolism of nutrients in culture media at a rate greater than that required for consumption
lipid peroxidation

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