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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
The human fibroplast is a classical example of

established cell lines
stable primary cell lines
none of these
cell transformations

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

They act as growth promoters
They act as growth inhibitors
Have no effect on cells
Lactate helps in the growth while ammonium inhibits the growth

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

increase in pH
reduction in the pH of culture hence loss of cell viability
no loss of cell viability
no change in pH

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

0.05-5 pmol of O2/cell/h
5 - 10 pmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
0.05-5 nmol of O2/cell/h

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Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

phenotypic modifications of cells in culture
release of genetic information
uptake of new genetic material
both (a) and (b)

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

solid medium
liquid medium
semi-solid medium
both (a) and (b)

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