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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
The human fibroplast is a classical example of

none of these
established cell lines
stable primary cell lines
cell transformations

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

enzymatic digestion
treating with chelating agents
physical disruption
All of these

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Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

All of these
Cytokines
Growth factors
Glucose and Glutamine

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

Lactate helps in the growth while ammonium inhibits the growth
They act as growth inhibitors
They act as growth promoters
Have no effect on cells

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Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

15-20%
2-5%
10-15%
1-10%

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

300-325
150-300
280-360
360-400

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