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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
The human fibroplast is a classical example of

cell transformations
stable primary cell lines
established cell lines
none of these

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

0.05-5 nmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
5 - 10 pmol of O2/cell/h
0.05-5 pmol of O2/cell/h

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

incomplete glutamine oxidation
increase in specific lactate production from glucose
both (a) and (b)
accumulation of ammonia

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

both (a) and (b)
liquid medium
solid medium
semi-solid medium

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

presence of bicarbonate buffer
addition of bases
none of these
presence of CO2

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Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Growth factors
Glucose and Glutamine
All of these
Cytokines

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