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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
The human fibroplast is a classical example of

cell transformations
established cell lines
none of these
stable primary cell lines

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

high glucose concentration is required
high glutamine concentration is required
low glucose concentration is required
low glutamine concentration is required

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Lactic acid
Glucose
ATP
Creatine phosphate

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Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

Whole embryo cultures
Organ cultures on plasma clots
All of these
Organ cultures on agar

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

lipid peroxidation
DNA degradation
All of these
metabolism of nutrients in culture media at a rate greater than that required for consumption

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

0.05-5 pmol of O2/cell/h
0.05-5 nmol of O2/cell/h
5 - 10 pmol of O2/cell/h
5 - 10 nmol of O2 /cell/h

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