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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
The human fibroplast is a classical example of

established cell lines
stable primary cell lines
none of these
cell transformations

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

0.05-5 pmol of O2/cell/h
5 - 10 pmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
0.05-5 nmol of O2/cell/h

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

high glucose concentration is required
low glucose concentration is required
low glutamine concentration is required
high glutamine concentration is required

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

They act as growth promoters
Lactate helps in the growth while ammonium inhibits the growth
They act as growth inhibitors
Have no effect on cells

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Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

All of these
Organ cultures on plasma clots
Organ cultures on agar
Whole embryo cultures

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by substituting glutamine by glutamate
All of these
by controlled addition of glutamine at low level
by removal of ammonia or ammonium from culture medium

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