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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
The human fibroplast is a classical example of

stable primary cell lines
none of these
cell transformations
established cell lines

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

none of these
both (a) and (b)
anaerobic pathway
oxidative pathway

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

150-300
300-325
280-360
360-400

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

ATP
Lactic acid
Creatine phosphate
Glucose

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

Helps in the cells respiration
All of these
It participates in the de novo synthesis of purines and pyrimidines
For monitoring pH of the culture

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Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

15-20%
10-15%
2-5%
1-10%

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