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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
The human fibroplast is a classical example of

cell transformations
none of these
stable primary cell lines
established cell lines

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

semi-solid medium
liquid medium
both (a) and (b)
solid medium

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

enzymatic digestion
All of these
physical disruption
treating with chelating agents

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

no change in pH
reduction in the pH of culture hence loss of cell viability
increase in pH
no loss of cell viability

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

increase in specific lactate production from glucose
incomplete glutamine oxidation
accumulation of ammonia
both (a) and (b)

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

ATP
Creatine phosphate
Lactic acid
Glucose

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