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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
The human fibroplast is a classical example of

established cell lines
none of these
cell transformations
stable primary cell lines

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Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Growth factors
Cytokines
All of these
Glucose and Glutamine

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

high glucose concentration is required
low glutamine concentration is required
low glucose concentration is required
high glutamine concentration is required

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

ATP
Glucose
Lactic acid
Creatine phosphate

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

increase in specific lactate production from glucose
incomplete glutamine oxidation
accumulation of ammonia
both (a) and (b)

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

Helps in the cells respiration
For monitoring pH of the culture
All of these
It participates in the de novo synthesis of purines and pyrimidines

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