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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
The human fibroplast is a classical example of

none of these
stable primary cell lines
established cell lines
cell transformations

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

enzymatic digestion
treating with chelating agents
All of these
physical disruption

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

anaerobic pathway
oxidative pathway
none of these
both (a) and (b)

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Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Slide culture
Roller test tube culture
Carrel flask culture
Adherent primary culture

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

Have no effect on cells
They act as growth inhibitors
They act as growth promoters
Lactate helps in the growth while ammonium inhibits the growth

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

high glutamine concentration is required
high glucose concentration is required
low glucose concentration is required
low glutamine concentration is required

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