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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
The human fibroplast is a classical example of

stable primary cell lines
established cell lines
cell transformations
none of these

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Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

8 amino acids
10 amino acids
6 amino acids
12 amino acids

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Animal Cell Culture and Regulation
Excess CO2 suppress cell growth and productivity by

both (a) and (b)
altering intracellular pH by diffusing across cell membrane
altering pH of the medium
inhibiting respiration

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

no loss of cell viability
increase in pH
reduction in the pH of culture hence loss of cell viability
no change in pH

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

It participates in the de novo synthesis of purines and pyrimidines
Helps in the cells respiration
For monitoring pH of the culture
All of these

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Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

CO2 and lactate
Lactate and ammonium
Lactate only
CO2 and NH3

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