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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
The human fibroplast is a classical example of

stable primary cell lines
none of these
cell transformations
established cell lines

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Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

All of these
Cytokines
Growth factors
Glucose and Glutamine

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

metabolism of nutrients in culture media at a rate greater than that required for consumption
lipid peroxidation
DNA degradation
All of these

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

high glucose concentration is required
low glutamine concentration is required
low glucose concentration is required
high glutamine concentration is required

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

105-150 mmol/litre
75-105 mmol/litre
55 - 75 mmol/litre
5.5 - 55 mmol/litre

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Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Carrel flask culture
Adherent primary culture
Roller test tube culture
Slide culture

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