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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
The human fibroplast is a classical example of

stable primary cell lines
none of these
established cell lines
cell transformations

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

propagated cell lines
established cell lines
secondary cell lines
primary cell lines

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

presence of CO2
none of these
addition of bases
presence of bicarbonate buffer

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

liquid medium
both (a) and (b)
solid medium
semi-solid medium

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Animal Cell Culture and Regulation
Excess CO2 suppress cell growth and productivity by

altering pH of the medium
inhibiting respiration
altering intracellular pH by diffusing across cell membrane
both (a) and (b)

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Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

release of genetic information
both (a) and (b)
phenotypic modifications of cells in culture
uptake of new genetic material

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