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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
The human fibroplast is a classical example of

stable primary cell lines
cell transformations
none of these
established cell lines

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Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Glucose and Glutamine
Growth factors
All of these
Cytokines

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Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

10-15%
1-10%
2-5%
15-20%

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Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

8 amino acids
6 amino acids
12 amino acids
10 amino acids

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

Lactate helps in the growth while ammonium inhibits the growth
They act as growth promoters
Have no effect on cells
They act as growth inhibitors

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

150-300
280-360
360-400
300-325

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