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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
The human fibroplast is a classical example of

cell transformations
stable primary cell lines
established cell lines
none of these

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

For monitoring pH of the culture
All of these
It participates in the de novo synthesis of purines and pyrimidines
Helps in the cells respiration

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

stable
anchorage independent
anchorage dependent
unstable

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

360-400
280-360
300-325
150-300

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

Lactate helps in the growth while ammonium inhibits the growth
They act as growth promoters
Have no effect on cells
They act as growth inhibitors

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

All of these
treating with chelating agents
enzymatic digestion
physical disruption

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