Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

established cell lines
primary cell lines
secondary cell lines
propagated cell lines

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Animal Cell Culture and Regulation
An established cell line can be called where it has been subcultured at least

70 times at an interval of 3 days between subcultures
50 times at an interval of 3 days between subcultures
40 times at an interval of 3 days between subcultures
70 times at an interval of 1 day between subcultures

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Animal Cell Culture and Regulation
The major problem associated with the isolation of free cells and cell aggregates from organs is that of

inhibiting the cells from their supporting matrix
None of these
releasing the cells from their supporting matrix
disintegrating the cells from their supporting matrix

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