Animal Cell Culture and Regulation Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called primary cell lines secondary cell lines established cell lines propagated cell lines primary cell lines secondary cell lines established cell lines propagated cell lines ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Range of optimum glucose concentration present in the culture media is 5.5 - 55 mmol/litre 105-150 mmol/litre 55 - 75 mmol/litre 75-105 mmol/litre 5.5 - 55 mmol/litre 105-150 mmol/litre 55 - 75 mmol/litre 75-105 mmol/litre ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation pH of culture medium is initially controlled by presence of CO2 addition of bases presence of bicarbonate buffer none of these presence of CO2 addition of bases presence of bicarbonate buffer none of these ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Accumulation of lactate leads to reduction in the pH of culture hence loss of cell viability no loss of cell viability increase in pH no change in pH reduction in the pH of culture hence loss of cell viability no loss of cell viability increase in pH no change in pH ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells? Lactate helps in the growth while ammonium inhibits the growth They act as growth inhibitors They act as growth promoters Have no effect on cells Lactate helps in the growth while ammonium inhibits the growth They act as growth inhibitors They act as growth promoters Have no effect on cells ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation How does CO2 help in the cell metabolism during cell culture? It participates in the de novo synthesis of purines and pyrimidines All of these Helps in the cells respiration For monitoring pH of the culture It participates in the de novo synthesis of purines and pyrimidines All of these Helps in the cells respiration For monitoring pH of the culture ANSWER DOWNLOAD EXAMIANS APP
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