Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

secondary cell lines
primary cell lines
propagated cell lines
established cell lines

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Animal Cell Culture and Regulation
During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture?

The cells have too much oxygen
Glycolysis is being inhibited
The cells do not have enough oxygen
Ethyl alcohol is being produced in excess

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