Animal Cell Culture and Regulation Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called established cell lines primary cell lines propagated cell lines secondary cell lines established cell lines primary cell lines propagated cell lines secondary cell lines ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is 300-325 150-300 360-400 280-360 300-325 150-300 360-400 280-360 ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Disaggregating of cells can be achieved by physical disruption enzymatic digestion treating with chelating agents All of these physical disruption enzymatic digestion treating with chelating agents All of these ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Toxicity due to accumulation of ammonia can be overcome by removal of ammonia or ammonium from culture medium by substituting glutamine by glutamate by controlled addition of glutamine at low level All of these by removal of ammonia or ammonium from culture medium by substituting glutamine by glutamate by controlled addition of glutamine at low level All of these ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells? Lactate helps in the growth while ammonium inhibits the growth They act as growth promoters Have no effect on cells They act as growth inhibitors Lactate helps in the growth while ammonium inhibits the growth They act as growth promoters Have no effect on cells They act as growth inhibitors ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Accumulation of lactate leads to no loss of cell viability no change in pH increase in pH reduction in the pH of culture hence loss of cell viability no loss of cell viability no change in pH increase in pH reduction in the pH of culture hence loss of cell viability ANSWER DOWNLOAD EXAMIANS APP
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