Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

primary cell lines
secondary cell lines
propagated cell lines
established cell lines

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Animal Cell Culture and Regulation
During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture?

Ethyl alcohol is being produced in excess
The cells do not have enough oxygen
Glycolysis is being inhibited
The cells have too much oxygen

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Animal Cell Culture and Regulation
The major problem associated with the isolation of free cells and cell aggregates from organs is that of

inhibiting the cells from their supporting matrix
None of these
disintegrating the cells from their supporting matrix
releasing the cells from their supporting matrix

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