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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

anchorage independent
unstable
anchorage dependent
stable

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

0.05-5 nmol of O2/cell/h
0.05-5 pmol of O2/cell/h
5 - 10 pmol of O2/cell/h
5 - 10 nmol of O2 /cell/h

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by removal of ammonia or ammonium from culture medium
All of these
by substituting glutamine by glutamate
by controlled addition of glutamine at low level

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Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

Organ cultures on agar
Organ cultures on plasma clots
Whole embryo cultures
All of these

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Creatine phosphate
ATP
Glucose
Lactic acid

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Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Adherent primary culture
Carrel flask culture
Roller test tube culture
Slide culture

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