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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

anchorage dependent
unstable
stable
anchorage independent

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

For monitoring pH of the culture
It participates in the de novo synthesis of purines and pyrimidines
All of these
Helps in the cells respiration

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Animal Cell Culture and Regulation
Excess CO2 suppress cell growth and productivity by

inhibiting respiration
both (a) and (b)
altering intracellular pH by diffusing across cell membrane
altering pH of the medium

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

ATP
Lactic acid
Glucose
Creatine phosphate

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Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

10-15%
15-20%
1-10%
2-5%

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Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

8 amino acids
10 amino acids
12 amino acids
6 amino acids

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