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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

stable
anchorage independent
anchorage dependent
unstable

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Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it forms a semi solid coagulum
it forms a clear and solid coagulum even after dilution
it doesn't produce solid clots
it is too opaque

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Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Glucose and Glutamine
Growth factors
Cytokines
All of these

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Glucose
Creatine phosphate
ATP
Lactic acid

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by controlled addition of glutamine at low level
by substituting glutamine by glutamate
by removal of ammonia or ammonium from culture medium
All of these

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

Helps in the cells respiration
It participates in the de novo synthesis of purines and pyrimidines
All of these
For monitoring pH of the culture

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