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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

unstable
anchorage dependent
anchorage independent
stable

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

1-2 mmol/litre
7-15 mmol/litre
2-7 mmol/litre
15 - 20 mmol/litre

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

no loss of cell viability
reduction in the pH of culture hence loss of cell viability
no change in pH
increase in pH

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Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

8 amino acids
6 amino acids
10 amino acids
12 amino acids

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

5 - 10 nmol of O2 /cell/h
0.05-5 pmol of O2/cell/h
5 - 10 pmol of O2/cell/h
0.05-5 nmol of O2/cell/h

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

increase in specific lactate production from glucose
accumulation of ammonia
both (a) and (b)
incomplete glutamine oxidation

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