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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

stable
anchorage dependent
anchorage independent
unstable

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Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it doesn't produce solid clots
it forms a clear and solid coagulum even after dilution
it is too opaque
it forms a semi solid coagulum

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

They act as growth inhibitors
They act as growth promoters
Have no effect on cells
Lactate helps in the growth while ammonium inhibits the growth

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Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

15-20%
1-10%
10-15%
2-5%

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

7-15 mmol/litre
2-7 mmol/litre
1-2 mmol/litre
15 - 20 mmol/litre

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

low glucose concentration is required
high glucose concentration is required
high glutamine concentration is required
low glutamine concentration is required

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