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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

unstable
anchorage dependent
stable
anchorage independent

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

Helps in the cells respiration
For monitoring pH of the culture
All of these
It participates in the de novo synthesis of purines and pyrimidines

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

addition of bases
presence of CO2
none of these
presence of bicarbonate buffer

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Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

8 amino acids
10 amino acids
12 amino acids
6 amino acids

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

propagated cell lines
primary cell lines
established cell lines
secondary cell lines

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

low glutamine concentration is required
low glucose concentration is required
high glutamine concentration is required
high glucose concentration is required

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