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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

unstable
anchorage independent
anchorage dependent
stable

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

metabolism of nutrients in culture media at a rate greater than that required for consumption
DNA degradation
lipid peroxidation
All of these

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

both (a) and (b)
anaerobic pathway
none of these
oxidative pathway

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

accumulation of ammonia
incomplete glutamine oxidation
both (a) and (b)
increase in specific lactate production from glucose

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

Lactate helps in the growth while ammonium inhibits the growth
They act as growth promoters
They act as growth inhibitors
Have no effect on cells

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Animal Cell Culture and Regulation
Excess CO2 suppress cell growth and productivity by

altering intracellular pH by diffusing across cell membrane
both (a) and (b)
altering pH of the medium
inhibiting respiration

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