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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

unstable
anchorage dependent
stable
anchorage independent

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

high glutamine concentration is required
high glucose concentration is required
low glucose concentration is required
low glutamine concentration is required

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by controlled addition of glutamine at low level
by substituting glutamine by glutamate
by removal of ammonia or ammonium from culture medium
All of these

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Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

phenotypic modifications of cells in culture
release of genetic information
uptake of new genetic material
both (a) and (b)

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Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

12 amino acids
10 amino acids
8 amino acids
6 amino acids

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

cell transformations
none of these
stable primary cell lines
established cell lines

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