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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

unstable
anchorage dependent
anchorage independent
stable

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

addition of bases
presence of CO2
none of these
presence of bicarbonate buffer

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Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Adherent primary culture
Carrel flask culture
Slide culture
Roller test tube culture

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

It participates in the de novo synthesis of purines and pyrimidines
Helps in the cells respiration
All of these
For monitoring pH of the culture

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Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

release of genetic information
uptake of new genetic material
both (a) and (b)
phenotypic modifications of cells in culture

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by substituting glutamine by glutamate
by controlled addition of glutamine at low level
by removal of ammonia or ammonium from culture medium
All of these

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