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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

unstable
anchorage independent
stable
anchorage dependent

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Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it is too opaque
it forms a clear and solid coagulum even after dilution
it doesn't produce solid clots
it forms a semi solid coagulum

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

For monitoring pH of the culture
Helps in the cells respiration
All of these
It participates in the de novo synthesis of purines and pyrimidines

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Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Growth factors
Glucose and Glutamine
Cytokines
All of these

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Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

1-10%
2-5%
15-20%
10-15%

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

1-2 mmol/litre
7-15 mmol/litre
15 - 20 mmol/litre
2-7 mmol/litre

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