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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

stable
anchorage independent
anchorage dependent
unstable

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

high glutamine concentration is required
high glucose concentration is required
low glucose concentration is required
low glutamine concentration is required

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Lactic acid
ATP
Creatine phosphate
Glucose

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

All of these
For monitoring pH of the culture
Helps in the cells respiration
It participates in the de novo synthesis of purines and pyrimidines

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

Lactate helps in the growth while ammonium inhibits the growth
They act as growth promoters
Have no effect on cells
They act as growth inhibitors

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

300-325
150-300
360-400
280-360

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