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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

anchorage independent
unstable
stable
anchorage dependent

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

15 - 20 mmol/litre
2-7 mmol/litre
1-2 mmol/litre
7-15 mmol/litre

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

DNA degradation
All of these
metabolism of nutrients in culture media at a rate greater than that required for consumption
lipid peroxidation

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Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

15-20%
10-15%
1-10%
2-5%

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by substituting glutamine by glutamate
by controlled addition of glutamine at low level
All of these
by removal of ammonia or ammonium from culture medium

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

high glutamine concentration is required
low glutamine concentration is required
high glucose concentration is required
low glucose concentration is required

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