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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

stable
anchorage independent
unstable
anchorage dependent

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

lipid peroxidation
All of these
metabolism of nutrients in culture media at a rate greater than that required for consumption
DNA degradation

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Animal Cell Culture and Regulation
Excess CO2 suppress cell growth and productivity by

inhibiting respiration
altering intracellular pH by diffusing across cell membrane
altering pH of the medium
both (a) and (b)

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

liquid medium
both (a) and (b)
solid medium
semi-solid medium

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

5 - 10 nmol of O2 /cell/h
0.05-5 nmol of O2/cell/h
5 - 10 pmol of O2/cell/h
0.05-5 pmol of O2/cell/h

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

reduction in the pH of culture hence loss of cell viability
increase in pH
no loss of cell viability
no change in pH

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