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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

anchorage independent
unstable
stable
anchorage dependent

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by substituting glutamine by glutamate
All of these
by controlled addition of glutamine at low level
by removal of ammonia or ammonium from culture medium

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Animal Cell Culture and Regulation
Excess CO2 suppress cell growth and productivity by

altering pH of the medium
both (a) and (b)
inhibiting respiration
altering intracellular pH by diffusing across cell membrane

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

lipid peroxidation
metabolism of nutrients in culture media at a rate greater than that required for consumption
DNA degradation
All of these

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Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

Lactate and ammonium
CO2 and NH3
Lactate only
CO2 and lactate

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

no loss of cell viability
reduction in the pH of culture hence loss of cell viability
no change in pH
increase in pH

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