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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

anchorage dependent
unstable
stable
anchorage independent

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Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

All of these
Whole embryo cultures
Organ cultures on agar
Organ cultures on plasma clots

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

All of these
physical disruption
treating with chelating agents
enzymatic digestion

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

75-105 mmol/litre
105-150 mmol/litre
5.5 - 55 mmol/litre
55 - 75 mmol/litre

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

oxidative pathway
none of these
both (a) and (b)
anaerobic pathway

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

5 - 10 pmol of O2/cell/h
0.05-5 pmol of O2/cell/h
0.05-5 nmol of O2/cell/h
5 - 10 nmol of O2 /cell/h

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