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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

stable
anchorage dependent
unstable
anchorage independent

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Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it forms a semi solid coagulum
it is too opaque
it forms a clear and solid coagulum even after dilution
it doesn't produce solid clots

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

both (a) and (b)
semi-solid medium
solid medium
liquid medium

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

primary cell lines
established cell lines
propagated cell lines
secondary cell lines

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

Helps in the cells respiration
It participates in the de novo synthesis of purines and pyrimidines
All of these
For monitoring pH of the culture

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

oxidative pathway
anaerobic pathway
both (a) and (b)
none of these

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