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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

anchorage dependent
stable
unstable
anchorage independent

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

reduction in the pH of culture hence loss of cell viability
no loss of cell viability
no change in pH
increase in pH

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Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

All of these
Cytokines
Glucose and Glutamine
Growth factors

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Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

1-10%
15-20%
10-15%
2-5%

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Creatine phosphate
Lactic acid
Glucose
ATP

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

accumulation of ammonia
increase in specific lactate production from glucose
incomplete glutamine oxidation
both (a) and (b)

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