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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

unstable
anchorage independent
anchorage dependent
stable

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Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

10 amino acids
6 amino acids
12 amino acids
8 amino acids

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

primary cell lines
propagated cell lines
established cell lines
secondary cell lines

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

presence of bicarbonate buffer
presence of CO2
none of these
addition of bases

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

0.05-5 nmol of O2/cell/h
0.05-5 pmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
5 - 10 pmol of O2/cell/h

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Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

Organ cultures on agar
Organ cultures on plasma clots
Whole embryo cultures
All of these

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