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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

anchorage independent
unstable
stable
anchorage dependent

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

addition of bases
presence of bicarbonate buffer
presence of CO2
none of these

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

anaerobic pathway
both (a) and (b)
none of these
oxidative pathway

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

accumulation of ammonia
both (a) and (b)
incomplete glutamine oxidation
increase in specific lactate production from glucose

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

150-300
300-325
360-400
280-360

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Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

Organ cultures on plasma clots
Organ cultures on agar
Whole embryo cultures
All of these

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