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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

unstable
stable
anchorage dependent
anchorage independent

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

no loss of cell viability
no change in pH
reduction in the pH of culture hence loss of cell viability
increase in pH

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

5 - 10 pmol of O2/cell/h
0.05-5 nmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
0.05-5 pmol of O2/cell/h

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

anaerobic pathway
oxidative pathway
none of these
both (a) and (b)

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

2-7 mmol/litre
1-2 mmol/litre
15 - 20 mmol/litre
7-15 mmol/litre

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Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

phenotypic modifications of cells in culture
release of genetic information
both (a) and (b)
uptake of new genetic material

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