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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

anchorage dependent
anchorage independent
stable
unstable

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Animal Cell Culture and Regulation
Excess CO2 suppress cell growth and productivity by

altering intracellular pH by diffusing across cell membrane
both (a) and (b)
inhibiting respiration
altering pH of the medium

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Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

2-5%
1-10%
10-15%
15-20%

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Lactic acid
Glucose
ATP
Creatine phosphate

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

All of these
metabolism of nutrients in culture media at a rate greater than that required for consumption
lipid peroxidation
DNA degradation

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

accumulation of ammonia
incomplete glutamine oxidation
increase in specific lactate production from glucose
both (a) and (b)

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