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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

anchorage independent
unstable
anchorage dependent
stable

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Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Carrel flask culture
Adherent primary culture
Roller test tube culture
Slide culture

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

7-15 mmol/litre
2-7 mmol/litre
1-2 mmol/litre
15 - 20 mmol/litre

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

semi-solid medium
both (a) and (b)
liquid medium
solid medium

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Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it doesn't produce solid clots
it forms a semi solid coagulum
it is too opaque
it forms a clear and solid coagulum even after dilution

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

5 - 10 pmol of O2/cell/h
0.05-5 nmol of O2/cell/h
0.05-5 pmol of O2/cell/h
5 - 10 nmol of O2 /cell/h

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