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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

anchorage dependent
unstable
stable
anchorage independent

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Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Roller test tube culture
Adherent primary culture
Slide culture
Carrel flask culture

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

1-2 mmol/litre
7-15 mmol/litre
2-7 mmol/litre
15 - 20 mmol/litre

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

increase in pH
no loss of cell viability
no change in pH
reduction in the pH of culture hence loss of cell viability

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

both (a) and (b)
none of these
oxidative pathway
anaerobic pathway

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

Have no effect on cells
They act as growth inhibitors
Lactate helps in the growth while ammonium inhibits the growth
They act as growth promoters

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