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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

anchorage dependent
stable
anchorage independent
unstable

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

280-360
360-400
150-300
300-325

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

presence of bicarbonate buffer
presence of CO2
none of these
addition of bases

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

established cell lines
none of these
stable primary cell lines
cell transformations

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

0.05-5 pmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
0.05-5 nmol of O2/cell/h
5 - 10 pmol of O2/cell/h

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

15 - 20 mmol/litre
2-7 mmol/litre
7-15 mmol/litre
1-2 mmol/litre

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