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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

anchorage independent
anchorage dependent
stable
unstable

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Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Growth factors
Glucose and Glutamine
All of these
Cytokines

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

established cell lines
stable primary cell lines
none of these
cell transformations

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

both (a) and (b)
incomplete glutamine oxidation
accumulation of ammonia
increase in specific lactate production from glucose

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

280-360
150-300
360-400
300-325

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

It participates in the de novo synthesis of purines and pyrimidines
Helps in the cells respiration
All of these
For monitoring pH of the culture

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