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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

anchorage dependent
stable
unstable
anchorage independent

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

both (a) and (b)
increase in specific lactate production from glucose
incomplete glutamine oxidation
accumulation of ammonia

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

both (a) and (b)
oxidative pathway
none of these
anaerobic pathway

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

treating with chelating agents
physical disruption
All of these
enzymatic digestion

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by substituting glutamine by glutamate
All of these
by removal of ammonia or ammonium from culture medium
by controlled addition of glutamine at low level

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

7-15 mmol/litre
1-2 mmol/litre
2-7 mmol/litre
15 - 20 mmol/litre

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