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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

treating with chelating agents
enzymatic digestion
physical disruption
All of these

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

It participates in the de novo synthesis of purines and pyrimidines
Helps in the cells respiration
For monitoring pH of the culture
All of these

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

5 - 10 pmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
0.05-5 pmol of O2/cell/h
0.05-5 nmol of O2/cell/h

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

accumulation of ammonia
increase in specific lactate production from glucose
incomplete glutamine oxidation
both (a) and (b)

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Animal Cell Culture and Regulation
Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping?

Turner's syndrome
all of these
Klinefelter's syndrome
Down's syndrome

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

1-2 mmol/litre
2-7 mmol/litre
7-15 mmol/litre
15 - 20 mmol/litre

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