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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

physical disruption
treating with chelating agents
enzymatic digestion
All of these

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

Lactate helps in the growth while ammonium inhibits the growth
They act as growth promoters
Have no effect on cells
They act as growth inhibitors

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

anchorage dependent
anchorage independent
unstable
stable

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

cell transformations
none of these
stable primary cell lines
established cell lines

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Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

release of genetic information
uptake of new genetic material
both (a) and (b)
phenotypic modifications of cells in culture

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

5 - 10 pmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
0.05-5 pmol of O2/cell/h
0.05-5 nmol of O2/cell/h

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