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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

treating with chelating agents
physical disruption
enzymatic digestion
All of these

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

lipid peroxidation
DNA degradation
metabolism of nutrients in culture media at a rate greater than that required for consumption
All of these

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

accumulation of ammonia
incomplete glutamine oxidation
both (a) and (b)
increase in specific lactate production from glucose

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

no loss of cell viability
no change in pH
reduction in the pH of culture hence loss of cell viability
increase in pH

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Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

6 amino acids
10 amino acids
8 amino acids
12 amino acids

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Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

All of these
Organ cultures on plasma clots
Organ cultures on agar
Whole embryo cultures

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