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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

physical disruption
treating with chelating agents
All of these
enzymatic digestion

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Animal Cell Culture and Regulation
Excess CO2 suppress cell growth and productivity by

both (a) and (b)
altering pH of the medium
altering intracellular pH by diffusing across cell membrane
inhibiting respiration

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Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

1-10%
2-5%
10-15%
15-20%

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Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

All of these
Organ cultures on plasma clots
Organ cultures on agar
Whole embryo cultures

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

For monitoring pH of the culture
All of these
Helps in the cells respiration
It participates in the de novo synthesis of purines and pyrimidines

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Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

phenotypic modifications of cells in culture
uptake of new genetic material
release of genetic information
both (a) and (b)

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