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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

physical disruption
All of these
treating with chelating agents
enzymatic digestion

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by substituting glutamine by glutamate
by removal of ammonia or ammonium from culture medium
by controlled addition of glutamine at low level
All of these

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

none of these
addition of bases
presence of CO2
presence of bicarbonate buffer

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

ATP
Lactic acid
Glucose
Creatine phosphate

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

75-105 mmol/litre
55 - 75 mmol/litre
5.5 - 55 mmol/litre
105-150 mmol/litre

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Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it forms a semi solid coagulum
it is too opaque
it forms a clear and solid coagulum even after dilution
it doesn't produce solid clots

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