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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

enzymatic digestion
physical disruption
All of these
treating with chelating agents

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Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it is too opaque
it doesn't produce solid clots
it forms a semi solid coagulum
it forms a clear and solid coagulum even after dilution

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Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

Organ cultures on agar
All of these
Whole embryo cultures
Organ cultures on plasma clots

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Creatine phosphate
Glucose
Lactic acid
ATP

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Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

Lactate only
CO2 and lactate
CO2 and NH3
Lactate and ammonium

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

300-325
280-360
150-300
360-400

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