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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

enzymatic digestion
treating with chelating agents
physical disruption
All of these

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Creatine phosphate
ATP
Lactic acid
Glucose

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

low glutamine concentration is required
high glutamine concentration is required
low glucose concentration is required
high glucose concentration is required

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

cell transformations
none of these
stable primary cell lines
established cell lines

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Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Cytokines
Growth factors
Glucose and Glutamine
All of these

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

lipid peroxidation
metabolism of nutrients in culture media at a rate greater than that required for consumption
DNA degradation
All of these

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