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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

enzymatic digestion
All of these
treating with chelating agents
physical disruption

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

solid medium
semi-solid medium
both (a) and (b)
liquid medium

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Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

All of these
Organ cultures on plasma clots
Organ cultures on agar
Whole embryo cultures

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Animal Cell Culture and Regulation
Excess CO2 suppress cell growth and productivity by

inhibiting respiration
both (a) and (b)
altering pH of the medium
altering intracellular pH by diffusing across cell membrane

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Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

All of these
Glucose and Glutamine
Cytokines
Growth factors

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

280-360
150-300
300-325
360-400

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