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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

All of these
treating with chelating agents
enzymatic digestion
physical disruption

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

All of these
by controlled addition of glutamine at low level
by removal of ammonia or ammonium from culture medium
by substituting glutamine by glutamate

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

none of these
addition of bases
presence of bicarbonate buffer
presence of CO2

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

105-150 mmol/litre
5.5 - 55 mmol/litre
55 - 75 mmol/litre
75-105 mmol/litre

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Creatine phosphate
ATP
Lactic acid
Glucose

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Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

release of genetic information
phenotypic modifications of cells in culture
both (a) and (b)
uptake of new genetic material

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