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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

physical disruption
enzymatic digestion
treating with chelating agents
All of these

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Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

Whole embryo cultures
Organ cultures on agar
Organ cultures on plasma clots
All of these

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Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

CO2 and lactate
CO2 and NH3
Lactate only
Lactate and ammonium

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Lactic acid
ATP
Creatine phosphate
Glucose

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

5 - 10 nmol of O2 /cell/h
5 - 10 pmol of O2/cell/h
0.05-5 pmol of O2/cell/h
0.05-5 nmol of O2/cell/h

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

propagated cell lines
secondary cell lines
established cell lines
primary cell lines

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