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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

treating with chelating agents
enzymatic digestion
All of these
physical disruption

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

55 - 75 mmol/litre
105-150 mmol/litre
5.5 - 55 mmol/litre
75-105 mmol/litre

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

solid medium
both (a) and (b)
semi-solid medium
liquid medium

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

anchorage dependent
anchorage independent
unstable
stable

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Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

Lactate and ammonium
CO2 and lactate
CO2 and NH3
Lactate only

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Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

1-10%
10-15%
2-5%
15-20%

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