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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

All of these
enzymatic digestion
treating with chelating agents
physical disruption

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

secondary cell lines
propagated cell lines
primary cell lines
established cell lines

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Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

12 amino acids
10 amino acids
8 amino acids
6 amino acids

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Lactic acid
Glucose
ATP
Creatine phosphate

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Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

1-10%
15-20%
2-5%
10-15%

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

280-360
360-400
150-300
300-325

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