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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

All of these
treating with chelating agents
physical disruption
enzymatic digestion

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by removal of ammonia or ammonium from culture medium
by substituting glutamine by glutamate
All of these
by controlled addition of glutamine at low level

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

established cell lines
stable primary cell lines
cell transformations
none of these

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

both (a) and (b)
solid medium
liquid medium
semi-solid medium

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

anchorage dependent
stable
unstable
anchorage independent

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Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

Whole embryo cultures
Organ cultures on agar
All of these
Organ cultures on plasma clots

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