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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

treating with chelating agents
enzymatic digestion
All of these
physical disruption

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

low glutamine concentration is required
high glucose concentration is required
high glutamine concentration is required
low glucose concentration is required

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

They act as growth inhibitors
Have no effect on cells
Lactate helps in the growth while ammonium inhibits the growth
They act as growth promoters

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

All of these
For monitoring pH of the culture
Helps in the cells respiration
It participates in the de novo synthesis of purines and pyrimidines

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

5 - 10 pmol of O2/cell/h
0.05-5 nmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
0.05-5 pmol of O2/cell/h

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Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

12 amino acids
6 amino acids
10 amino acids
8 amino acids

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