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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

physical disruption
All of these
treating with chelating agents
enzymatic digestion

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Creatine phosphate
Glucose
Lactic acid
ATP

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

both (a) and (b)
none of these
anaerobic pathway
oxidative pathway

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

1-2 mmol/litre
2-7 mmol/litre
7-15 mmol/litre
15 - 20 mmol/litre

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

stable
unstable
anchorage dependent
anchorage independent

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Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Glucose and Glutamine
Growth factors
Cytokines
All of these

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