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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

enzymatic digestion
physical disruption
treating with chelating agents
All of these

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

primary cell lines
secondary cell lines
propagated cell lines
established cell lines

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Animal Cell Culture and Regulation
Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping?

Klinefelter's syndrome
all of these
Down's syndrome
Turner's syndrome

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by controlled addition of glutamine at low level
All of these
by substituting glutamine by glutamate
by removal of ammonia or ammonium from culture medium

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

both (a) and (b)
none of these
oxidative pathway
anaerobic pathway

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Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

10 amino acids
6 amino acids
8 amino acids
12 amino acids

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