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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

All of these
enzymatic digestion
treating with chelating agents
physical disruption

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

360-400
300-325
150-300
280-360

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

high glucose concentration is required
low glucose concentration is required
high glutamine concentration is required
low glutamine concentration is required

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Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

All of these
Growth factors
Cytokines
Glucose and Glutamine

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Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Adherent primary culture
Slide culture
Carrel flask culture
Roller test tube culture

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by removal of ammonia or ammonium from culture medium
by substituting glutamine by glutamate
by controlled addition of glutamine at low level
All of these

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