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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

enzymatic digestion
All of these
physical disruption
treating with chelating agents

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Animal Cell Culture and Regulation
Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping?

all of these
Klinefelter's syndrome
Turner's syndrome
Down's syndrome

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

both (a) and (b)
accumulation of ammonia
incomplete glutamine oxidation
increase in specific lactate production from glucose

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by removal of ammonia or ammonium from culture medium
All of these
by substituting glutamine by glutamate
by controlled addition of glutamine at low level

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

1-2 mmol/litre
15 - 20 mmol/litre
7-15 mmol/litre
2-7 mmol/litre

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

anchorage independent
anchorage dependent
unstable
stable

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