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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

All of these
enzymatic digestion
treating with chelating agents
physical disruption

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

unstable
anchorage dependent
stable
anchorage independent

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

presence of bicarbonate buffer
none of these
addition of bases
presence of CO2

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

DNA degradation
All of these
lipid peroxidation
metabolism of nutrients in culture media at a rate greater than that required for consumption

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

established cell lines
stable primary cell lines
none of these
cell transformations

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by removal of ammonia or ammonium from culture medium
by controlled addition of glutamine at low level
by substituting glutamine by glutamate
All of these

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