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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

treating with chelating agents
physical disruption
All of these
enzymatic digestion

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Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

phenotypic modifications of cells in culture
uptake of new genetic material
both (a) and (b)
release of genetic information

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Animal Cell Culture and Regulation
Range of optimum glutamine concentration present in the culture media is

15 - 20 mmol/litre
2-7 mmol/litre
7-15 mmol/litre
1-2 mmol/litre

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Lactic acid
ATP
Creatine phosphate
Glucose

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

360-400
300-325
280-360
150-300

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Animal Cell Culture and Regulation
Which of the following is not the explantation technique?

Slide culture
Roller test tube culture
Adherent primary culture
Carrel flask culture

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