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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

physical disruption
treating with chelating agents
enzymatic digestion
All of these

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Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it doesn't produce solid clots
it forms a semi solid coagulum
it is too opaque
it forms a clear and solid coagulum even after dilution

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

anchorage dependent
anchorage independent
unstable
stable

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

addition of bases
presence of bicarbonate buffer
presence of CO2
none of these

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Lactic acid
Glucose
ATP
Creatine phosphate

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

They act as growth inhibitors
They act as growth promoters
Lactate helps in the growth while ammonium inhibits the growth
Have no effect on cells

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