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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

physical disruption
enzymatic digestion
All of these
treating with chelating agents

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

high glutamine concentration is required
high glucose concentration is required
low glucose concentration is required
low glutamine concentration is required

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

360-400
300-325
150-300
280-360

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Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

Organ cultures on agar
All of these
Whole embryo cultures
Organ cultures on plasma clots

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Animal Cell Culture and Regulation
Excess CO2 suppress cell growth and productivity by

altering pH of the medium
altering intracellular pH by diffusing across cell membrane
both (a) and (b)
inhibiting respiration

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

presence of bicarbonate buffer
presence of CO2
none of these
addition of bases

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