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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

physical disruption
enzymatic digestion
treating with chelating agents
All of these

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Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it doesn't produce solid clots
it is too opaque
it forms a clear and solid coagulum even after dilution
it forms a semi solid coagulum

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

DNA degradation
metabolism of nutrients in culture media at a rate greater than that required for consumption
All of these
lipid peroxidation

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

presence of bicarbonate buffer
none of these
presence of CO2
addition of bases

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

secondary cell lines
propagated cell lines
primary cell lines
established cell lines

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

no change in pH
no loss of cell viability
increase in pH
reduction in the pH of culture hence loss of cell viability

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