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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

All of these
treating with chelating agents
physical disruption
enzymatic digestion

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

low glutamine concentration is required
high glutamine concentration is required
low glucose concentration is required
high glucose concentration is required

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

55 - 75 mmol/litre
75-105 mmol/litre
5.5 - 55 mmol/litre
105-150 mmol/litre

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Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

phenotypic modifications of cells in culture
both (a) and (b)
release of genetic information
uptake of new genetic material

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

established cell lines
secondary cell lines
primary cell lines
propagated cell lines

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Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Growth factors
Glucose and Glutamine
All of these
Cytokines

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