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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

physical disruption
enzymatic digestion
All of these
treating with chelating agents

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

ATP
Glucose
Creatine phosphate
Lactic acid

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

All of these
by removal of ammonia or ammonium from culture medium
by substituting glutamine by glutamate
by controlled addition of glutamine at low level

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

accumulation of ammonia
both (a) and (b)
incomplete glutamine oxidation
increase in specific lactate production from glucose

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

Helps in the cells respiration
For monitoring pH of the culture
It participates in the de novo synthesis of purines and pyrimidines
All of these

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Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

Lactate and ammonium
CO2 and NH3
CO2 and lactate
Lactate only

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