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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

physical disruption
treating with chelating agents
enzymatic digestion
All of these

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

300-325
150-300
280-360
360-400

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

lipid peroxidation
All of these
DNA degradation
metabolism of nutrients in culture media at a rate greater than that required for consumption

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

Have no effect on cells
Lactate helps in the growth while ammonium inhibits the growth
They act as growth inhibitors
They act as growth promoters

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

unstable
stable
anchorage dependent
anchorage independent

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

secondary cell lines
established cell lines
propagated cell lines
primary cell lines

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