Animal Cell Culture and Regulation An established cell line can be called where it has been subcultured at least 70 times at an interval of 3 days between subcultures 40 times at an interval of 3 days between subcultures 70 times at an interval of 1 day between subcultures 50 times at an interval of 3 days between subcultures 70 times at an interval of 3 days between subcultures 40 times at an interval of 3 days between subcultures 70 times at an interval of 1 day between subcultures 50 times at an interval of 3 days between subcultures ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Toxicity due to accumulation of ammonia can be overcome All of these by removal of ammonia or ammonium from culture medium by controlled addition of glutamine at low level by substituting glutamine by glutamate All of these by removal of ammonia or ammonium from culture medium by controlled addition of glutamine at low level by substituting glutamine by glutamate ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Higher dissolved oxygen concentration in the culture media are toxic and lead to DNA degradation lipid peroxidation All of these metabolism of nutrients in culture media at a rate greater than that required for consumption DNA degradation lipid peroxidation All of these metabolism of nutrients in culture media at a rate greater than that required for consumption ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Cells which have undergone transformation frequently become unstable anchorage dependent anchorage independent stable unstable anchorage dependent anchorage independent stable ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called primary cell lines established cell lines secondary cell lines propagated cell lines primary cell lines established cell lines secondary cell lines propagated cell lines ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of 10 amino acids 8 amino acids 12 amino acids 6 amino acids 10 amino acids 8 amino acids 12 amino acids 6 amino acids ANSWER DOWNLOAD EXAMIANS APP