Animal Cell Culture and Regulation An established cell line can be called where it has been subcultured at least 50 times at an interval of 3 days between subcultures 70 times at an interval of 1 day between subcultures 70 times at an interval of 3 days between subcultures 40 times at an interval of 3 days between subcultures 50 times at an interval of 3 days between subcultures 70 times at an interval of 1 day between subcultures 70 times at an interval of 3 days between subcultures 40 times at an interval of 3 days between subcultures ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is 150-300 300-325 360-400 280-360 150-300 300-325 360-400 280-360 ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture? The cells have too much oxygen The cells do not have enough oxygen Ethyl alcohol is being produced in excess Glycolysis is being inhibited The cells have too much oxygen The cells do not have enough oxygen Ethyl alcohol is being produced in excess Glycolysis is being inhibited ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation How does CO2 help in the cell metabolism during cell culture? All of these Helps in the cells respiration For monitoring pH of the culture It participates in the de novo synthesis of purines and pyrimidines All of these Helps in the cells respiration For monitoring pH of the culture It participates in the de novo synthesis of purines and pyrimidines ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Higher dissolved oxygen concentration in the culture media are toxic and lead to DNA degradation metabolism of nutrients in culture media at a rate greater than that required for consumption All of these lipid peroxidation DNA degradation metabolism of nutrients in culture media at a rate greater than that required for consumption All of these lipid peroxidation ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation To prevent the accumulation of lactate low glucose concentration is required high glutamine concentration is required low glutamine concentration is required high glucose concentration is required low glucose concentration is required high glutamine concentration is required low glutamine concentration is required high glucose concentration is required ANSWER DOWNLOAD EXAMIANS APP
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