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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

6 amino acids
10 amino acids
12 amino acids
8 amino acids

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

high glutamine concentration is required
high glucose concentration is required
low glutamine concentration is required
low glucose concentration is required

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

established cell lines
secondary cell lines
primary cell lines
propagated cell lines

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

none of these
presence of CO2
addition of bases
presence of bicarbonate buffer

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Lactic acid
ATP
Creatine phosphate
Glucose

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

no change in pH
no loss of cell viability
increase in pH
reduction in the pH of culture hence loss of cell viability

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