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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

10 amino acids
6 amino acids
12 amino acids
8 amino acids

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Animal Cell Culture and Regulation
Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping?

all of these
Klinefelter's syndrome
Turner's syndrome
Down's syndrome

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Animal Cell Culture and Regulation
Range of optimum glucose concentration present in the culture media is

5.5 - 55 mmol/litre
75-105 mmol/litre
105-150 mmol/litre
55 - 75 mmol/litre

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by substituting glutamine by glutamate
by controlled addition of glutamine at low level
All of these
by removal of ammonia or ammonium from culture medium

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

incomplete glutamine oxidation
both (a) and (b)
increase in specific lactate production from glucose
accumulation of ammonia

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

physical disruption
All of these
treating with chelating agents
enzymatic digestion

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