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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

6 amino acids
12 amino acids
10 amino acids
8 amino acids

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

All of these
by substituting glutamine by glutamate
by controlled addition of glutamine at low level
by removal of ammonia or ammonium from culture medium

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Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

CO2 and lactate
CO2 and NH3
Lactate only
Lactate and ammonium

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Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

both (a) and (b)
oxidative pathway
none of these
anaerobic pathway

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

solid medium
liquid medium
both (a) and (b)
semi-solid medium

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

increase in pH
reduction in the pH of culture hence loss of cell viability
no loss of cell viability
no change in pH

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