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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

12 amino acids
6 amino acids
10 amino acids
8 amino acids

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Animal Cell Culture and Regulation
The human fibroplast is a classical example of

cell transformations
stable primary cell lines
none of these
established cell lines

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by controlled addition of glutamine at low level
by substituting glutamine by glutamate
by removal of ammonia or ammonium from culture medium
All of these

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Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it is too opaque
it forms a semi solid coagulum
it doesn't produce solid clots
it forms a clear and solid coagulum even after dilution

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

semi-solid medium
solid medium
liquid medium
both (a) and (b)

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

high glucose concentration is required
low glucose concentration is required
high glutamine concentration is required
low glutamine concentration is required

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