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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

8 amino acids
10 amino acids
6 amino acids
12 amino acids

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

5 - 10 pmol of O2/cell/h
0.05-5 nmol of O2/cell/h
5 - 10 nmol of O2 /cell/h
0.05-5 pmol of O2/cell/h

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

increase in specific lactate production from glucose
incomplete glutamine oxidation
accumulation of ammonia
both (a) and (b)

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

increase in pH
no loss of cell viability
reduction in the pH of culture hence loss of cell viability
no change in pH

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

DNA degradation
All of these
metabolism of nutrients in culture media at a rate greater than that required for consumption
lipid peroxidation

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

treating with chelating agents
All of these
enzymatic digestion
physical disruption

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