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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

12 amino acids
8 amino acids
6 amino acids
10 amino acids

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Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

phenotypic modifications of cells in culture
uptake of new genetic material
both (a) and (b)
release of genetic information

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

All of these
physical disruption
treating with chelating agents
enzymatic digestion

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Animal Cell Culture and Regulation
pH of culture medium is initially controlled by

addition of bases
none of these
presence of bicarbonate buffer
presence of CO2

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Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it forms a clear and solid coagulum even after dilution
it doesn't produce solid clots
it forms a semi solid coagulum
it is too opaque

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

metabolism of nutrients in culture media at a rate greater than that required for consumption
DNA degradation
lipid peroxidation
All of these

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