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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

12 amino acids
6 amino acids
8 amino acids
10 amino acids

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Animal Cell Culture and Regulation
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

0.05-5 pmol of O2/cell/h
0.05-5 nmol of O2/cell/h
5 - 10 pmol of O2/cell/h
5 - 10 nmol of O2 /cell/h

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

280-360
360-400
300-325
150-300

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Animal Cell Culture and Regulation
Which of the followings are the metabolic products of glucose and glutamine?

Lactate only
CO2 and lactate
CO2 and NH3
Lactate and ammonium

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

physical disruption
treating with chelating agents
All of these
enzymatic digestion

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

All of these
by substituting glutamine by glutamate
by controlled addition of glutamine at low level
by removal of ammonia or ammonium from culture medium

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