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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

6 amino acids
10 amino acids
8 amino acids
12 amino acids

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

primary cell lines
propagated cell lines
secondary cell lines
established cell lines

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Animal Cell Culture and Regulation
The technique of organ culture may be divided on the basis of employing

both (a) and (b)
solid medium
liquid medium
semi-solid medium

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Animal Cell Culture and Regulation
What is the effect of excess accumulation of metabolite products (lactate and ammonium) on cells?

They act as growth promoters
Lactate helps in the growth while ammonium inhibits the growth
Have no effect on cells
They act as growth inhibitors

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by controlled addition of glutamine at low level
by substituting glutamine by glutamate
by removal of ammonia or ammonium from culture medium
All of these

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

DNA degradation
lipid peroxidation
metabolism of nutrients in culture media at a rate greater than that required for consumption
All of these

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