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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

10 amino acids
12 amino acids
8 amino acids
6 amino acids

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Animal Cell Culture and Regulation
Toxicity due to accumulation of ammonia can be overcome

by controlled addition of glutamine at low level
All of these
by removal of ammonia or ammonium from culture medium
by substituting glutamine by glutamate

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Animal Cell Culture and Regulation
How does CO2 help in the cell metabolism during cell culture?

It participates in the de novo synthesis of purines and pyrimidines
All of these
Helps in the cells respiration
For monitoring pH of the culture

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

stable
unstable
anchorage independent
anchorage dependent

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Animal Cell Culture and Regulation
Accumulation of lactate leads to

no loss of cell viability
reduction in the pH of culture hence loss of cell viability
increase in pH
no change in pH

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

secondary cell lines
established cell lines
primary cell lines
propagated cell lines

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