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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of

6 amino acids
12 amino acids
10 amino acids
8 amino acids

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

high glucose concentration is required
high glutamine concentration is required
low glucose concentration is required
low glutamine concentration is required

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Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

Whole embryo cultures
Organ cultures on plasma clots
All of these
Organ cultures on agar

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Animal Cell Culture and Regulation
Which of the following is not a source of energy in active muscle cells?

Glucose
Creatine phosphate
Lactic acid
ATP

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Animal Cell Culture and Regulation
When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

increase in specific lactate production from glucose
incomplete glutamine oxidation
both (a) and (b)
accumulation of ammonia

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Animal Cell Culture and Regulation
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

300-325
360-400
150-300
280-360

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