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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

none of these
both (a) and (b)
oxidative pathway
anaerobic pathway

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Animal Cell Culture and Regulation
To prevent the accumulation of lactate

high glutamine concentration is required
low glutamine concentration is required
low glucose concentration is required
high glucose concentration is required

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Animal Cell Culture and Regulation
For culturing, plasma from the adult chicken is preferred to mammalian plasma because

it forms a clear and solid coagulum even after dilution
it is too opaque
it doesn't produce solid clots
it forms a semi solid coagulum

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

DNA degradation
All of these
metabolism of nutrients in culture media at a rate greater than that required for consumption
lipid peroxidation

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Animal Cell Culture and Regulation
Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

established cell lines
primary cell lines
secondary cell lines
propagated cell lines

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Animal Cell Culture and Regulation
Which of the following is the technique used for the embryo culture?

All of these
Organ cultures on agar
Whole embryo cultures
Organ cultures on plasma clots

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