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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

oxidative pathway
both (a) and (b)
none of these
anaerobic pathway

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

unstable
anchorage independent
stable
anchorage dependent

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Animal Cell Culture and Regulation
What is the concentration of CO2 required for culturing animal cells?

15-20%
10-15%
2-5%
1-10%

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Animal Cell Culture and Regulation
Excess CO2 suppress cell growth and productivity by

inhibiting respiration
altering pH of the medium
both (a) and (b)
altering intracellular pH by diffusing across cell membrane

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Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

Cytokines
Glucose and Glutamine
All of these
Growth factors

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Animal Cell Culture and Regulation
Disaggregating of cells can be achieved by

enzymatic digestion
All of these
treating with chelating agents
physical disruption

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