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Animal Cell Culture and Regulation

Animal Cell Culture and Regulation
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

anaerobic pathway
none of these
oxidative pathway
both (a) and (b)

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Animal Cell Culture and Regulation
Cells which have undergone transformation frequently become

unstable
anchorage independent
anchorage dependent
stable

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Animal Cell Culture and Regulation
What are the main constituents of culture for animal cell growth?

All of these
Growth factors
Glucose and Glutamine
Cytokines

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Animal Cell Culture and Regulation
In animal cell culture, particularly mammalian cell culture, transformation means

release of genetic information
phenotypic modifications of cells in culture
both (a) and (b)
uptake of new genetic material

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Animal Cell Culture and Regulation
Excess CO2 suppress cell growth and productivity by

both (a) and (b)
altering intracellular pH by diffusing across cell membrane
inhibiting respiration
altering pH of the medium

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Animal Cell Culture and Regulation
Higher dissolved oxygen concentration in the culture media are toxic and lead to

All of these
lipid peroxidation
DNA degradation
metabolism of nutrients in culture media at a rate greater than that required for consumption

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