Animal Cell Culture and Regulation At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via none of these oxidative pathway both (a) and (b) anaerobic pathway none of these oxidative pathway both (a) and (b) anaerobic pathway ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation An established cell line can be called where it has been subcultured at least 70 times at an interval of 1 day between subcultures 70 times at an interval of 3 days between subcultures 40 times at an interval of 3 days between subcultures 50 times at an interval of 3 days between subcultures 70 times at an interval of 1 day between subcultures 70 times at an interval of 3 days between subcultures 40 times at an interval of 3 days between subcultures 50 times at an interval of 3 days between subcultures ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of accumulation of ammonia incomplete glutamine oxidation both (a) and (b) increase in specific lactate production from glucose accumulation of ammonia incomplete glutamine oxidation both (a) and (b) increase in specific lactate production from glucose ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation In animal cell culture, particularly mammalian cell culture, transformation means uptake of new genetic material both (a) and (b) phenotypic modifications of cells in culture release of genetic information uptake of new genetic material both (a) and (b) phenotypic modifications of cells in culture release of genetic information ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Toxicity due to accumulation of ammonia can be overcome All of these by controlled addition of glutamine at low level by substituting glutamine by glutamate by removal of ammonia or ammonium from culture medium All of these by controlled addition of glutamine at low level by substituting glutamine by glutamate by removal of ammonia or ammonium from culture medium ANSWER DOWNLOAD EXAMIANS APP
Animal Cell Culture and Regulation Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively) 5 - 10 nmol of O2 /cell/h 0.05-5 nmol of O2/cell/h 5 - 10 pmol of O2/cell/h 0.05-5 pmol of O2/cell/h 5 - 10 nmol of O2 /cell/h 0.05-5 nmol of O2/cell/h 5 - 10 pmol of O2/cell/h 0.05-5 pmol of O2/cell/h ANSWER DOWNLOAD EXAMIANS APP