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Cloning Vectors

Cloning Vectors
M 13 is an example of

filamentous phage
plasmid
both (a) and (b)
single stranded DNA vector

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Cloning Vectors
Which of the following is not true about phagemid?

Contain λ att site
Can only be propagated as phage
Contain functional origin of replication of the plasmid and λ phage
May be propagated as a plasmid or as phage in appropriate strain

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Cloning Vectors
Plasmids which are maintained as multiple copy number per cell are known as

stringent plasmids
none of these
cryptic plasmids
relaxed plasmids

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Cloning Vectors
P1 cloning vector is the example of

bacteriophage
phagemid
plasmid
cosmid

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Cloning Vectors
Stuffer is

the left arm of the vector DNA
the right arm of the vector DNA
None of these
central fragment of the vector DNA

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Cloning Vectors
A plasmid can be considered as a suitable cloning vector if

it possesses a single restriction site for one or more restriction enzymes
it can be readily isolated from the cells
insertion of foreign DNA does not alter its replication properties
All of these

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