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Cloning Vectors

Cloning Vectors
M 13 is an example of

both (a) and (b)
plasmid
single stranded DNA vector
filamentous phage

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Cloning Vectors
Which of the following is not true about phagemid?

May be propagated as a plasmid or as phage in appropriate strain
Contain λ att site
Contain functional origin of replication of the plasmid and λ phage
Can only be propagated as phage

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Cloning Vectors
P1 cloning vector allow cloning of DNA of the length of

20 kbp
100 kbp
50 kbp
10 kbp

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Cloning Vectors
Maximum size of foreign DNA that can be inserted into a replacement vector is

40-50 kb
20-25 kb
18-20 kb
25-30 kb

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Cloning Vectors
Maximum size of foreign DNA that can be inserted into an insertion vector is

50 kb
27 kb
35 kb
18 kb

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Cloning Vectors
Cosmid vectors are used for

cloning eukaryotic DNA only
cloning small fragments of DNA
cloning prokaryotic DNA only
cloning large fragments of DNA

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