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Cloning Vectors

Cloning Vectors
M 13 is an example of

both (a) and (b)
plasmid
single stranded DNA vector
filamentous phage

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Cloning Vectors
Cryptic plasmids

exhibit antibiotic resistance
exhibit one phenotypic traits
exhibit many phenotypic traits
do not exhibit any phenotypic trait

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Cloning Vectors
A plasmid can be considered as a suitable cloning vector if

All of these
it can be readily isolated from the cells
it possesses a single restriction site for one or more restriction enzymes
insertion of foreign DNA does not alter its replication properties

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Cloning Vectors
Single stranded vectors are useful

All of these
for probe preparation
for oligonucleotide directed mutagenesis
for sequencing of cloned DNA

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Cloning Vectors
Phagemid vectors are

combination of phages and cosmid
All of these
phages carrying properties of plasmids
combination of plasmid and phage λ

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Cloning Vectors
Maximum size of foreign DNA that can be inserted into a replacement vector is

25-30 kb
20-25 kb
40-50 kb
18-20 kb

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MORE MCQ ON Cloning Vectors

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