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Cloning Vectors

Cloning Vectors
P1 cloning vector allow cloning of DNA of the length of

10 kbp
100 kbp
50 kbp
20 kbp

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Cloning Vectors
Phagemid vectors are

All of these
phages carrying properties of plasmids
combination of phages and cosmid
combination of plasmid and phage λ

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Cloning Vectors
Single stranded vectors are useful

for sequencing of cloned DNA
All of these
for probe preparation
for oligonucleotide directed mutagenesis

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Cloning Vectors
λ ZAP vector is an example of

phage
phagemid
plasmid
cosmid

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Cloning Vectors
Which of the following is not true about phagemid?

May be propagated as a plasmid or as phage in appropriate strain
Contain λ att site
Contain functional origin of replication of the plasmid and λ phage
Can only be propagated as phage

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Cloning Vectors
Inserted DNA in λ gt 11 can be expressed as

free protein in the cytoplasm
β-galactosidase fused protein
All of these
free protein that is secreted out

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