Cloning Vectors P1 cloning vector allow cloning of DNA of the length of 100 kbp 20 kbp 10 kbp 50 kbp 100 kbp 20 kbp 10 kbp 50 kbp ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Which of the following is not true about phagemid? Contain λ att site Contain functional origin of replication of the plasmid and λ phage Can only be propagated as phage May be propagated as a plasmid or as phage in appropriate strain Contain λ att site Contain functional origin of replication of the plasmid and λ phage Can only be propagated as phage May be propagated as a plasmid or as phage in appropriate strain ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Cosmid vectors are plasmids that contain fragment of λ DNA including the cos site plasmids that have no selection marker phages that lack cos site cryptic plasmids plasmids that contain fragment of λ DNA including the cos site plasmids that have no selection marker phages that lack cos site cryptic plasmids ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Plasmid incompatibility is inability of two different plasmids to coexist in the same host cell in the absence of selection pressure. inability of a plasmid to grow in the host None of these both (a) and (b) inability of two different plasmids to coexist in the same host cell in the absence of selection pressure. inability of a plasmid to grow in the host None of these both (a) and (b) ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Cos site of the cosmids consists of 12 bases helps whole genome in circularization and ligation both (a) and (b) contains cleavage site consists of 12 bases helps whole genome in circularization and ligation both (a) and (b) contains cleavage site ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to 40-44 kb 6-7 kb 20-23 kb 1-2 kb 40-44 kb 6-7 kb 20-23 kb 1-2 kb ANSWER DOWNLOAD EXAMIANS APP