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Cloning Vectors

Cloning Vectors
P1 cloning vector allow cloning of DNA of the length of

20 kbp
100 kbp
10 kbp
50 kbp

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Cloning Vectors
Which of the following is not true about phagemid?

May be propagated as a plasmid or as phage in appropriate strain
Contain λ att site
Contain functional origin of replication of the plasmid and λ phage
Can only be propagated as phage

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Cloning Vectors
Difference between λ gt 10 and λ gt 11 vectors is that

λ gt 10 is an expression vector
λ gt 11 is an expression vector
λ gt 10 is a replacement vector
λ gt 11 is a replacement vector

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Cloning Vectors
Phagemid vectors are

All of these
phages carrying properties of plasmids
combination of phages and cosmid
combination of plasmid and phage λ

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Cloning Vectors
Maximum size of foreign DNA that can be inserted into a replacement vector is

40-50 kb
25-30 kb
18-20 kb
20-25 kb

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Cloning Vectors
Cosmid vectors are used for

cloning small fragments of DNA
cloning prokaryotic DNA only
cloning large fragments of DNA
cloning eukaryotic DNA only

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MORE MCQ ON Cloning Vectors

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