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Cloning Vectors

Cloning Vectors
P1 cloning vector allow cloning of DNA of the length of

50 kbp
20 kbp
10 kbp
100 kbp

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Cloning Vectors
Cosmid vectors are used for

cloning prokaryotic DNA only
cloning large fragments of DNA
cloning eukaryotic DNA only
cloning small fragments of DNA

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Cloning Vectors
A plasmid can be considered as a suitable cloning vector if

it can be readily isolated from the cells
it possesses a single restriction site for one or more restriction enzymes
insertion of foreign DNA does not alter its replication properties
All of these

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Cloning Vectors
Maximum size of foreign DNA that can be inserted into a replacement vector is

25-30 kb
20-25 kb
18-20 kb
40-50 kb

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Cloning Vectors
pBR 322 has/have which of the following selection marker(s)?

Kanr
Both (a) and (b)
Tetr
Ampr

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Cloning Vectors
Cos site of the cosmids

contains cleavage site
both (a) and (b)
helps whole genome in circularization and ligation
consists of 12 bases

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MORE MCQ ON Cloning Vectors

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