Cloning Vectors Cosmid vectors are used for cloning large fragments of DNA cloning eukaryotic DNA only cloning small fragments of DNA cloning prokaryotic DNA only cloning large fragments of DNA cloning eukaryotic DNA only cloning small fragments of DNA cloning prokaryotic DNA only ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors λ ZAP vector is an example of phage plasmid cosmid phagemid phage plasmid cosmid phagemid ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors A plasmid can be considered as a suitable cloning vector if it can be readily isolated from the cells insertion of foreign DNA does not alter its replication properties All of these it possesses a single restriction site for one or more restriction enzymes it can be readily isolated from the cells insertion of foreign DNA does not alter its replication properties All of these it possesses a single restriction site for one or more restriction enzymes ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Which of the following is not true about phagemid? Contain functional origin of replication of the plasmid and λ phage Can only be propagated as phage May be propagated as a plasmid or as phage in appropriate strain Contain λ att site Contain functional origin of replication of the plasmid and λ phage Can only be propagated as phage May be propagated as a plasmid or as phage in appropriate strain Contain λ att site ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Size of the DNA that can be packaged into a λ phage is any size 50 kb 40-50 kb 35-53 kb any size 50 kb 40-50 kb 35-53 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Cos site of the cosmids helps whole genome in circularization and ligation consists of 12 bases contains cleavage site both (a) and (b) helps whole genome in circularization and ligation consists of 12 bases contains cleavage site both (a) and (b) ANSWER DOWNLOAD EXAMIANS APP