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Cloning Vectors

Cloning Vectors
Charon 34 and Charon 35 can clone DNA upto

9-20 kb
30-35 kb
1-2 kb
6-7 kb

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Cloning Vectors
Maximum size of foreign DNA that can be inserted into a replacement vector is

20-25 kb
25-30 kb
18-20 kb
40-50 kb

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Cloning Vectors
EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to

1-2 kb
40-44 kb
6-7 kb
15-25 kb

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Cloning Vectors
Inserted DNA in λ gt 11 can be expressed as

β-galactosidase fused protein
free protein in the cytoplasm
free protein that is secreted out
All of these

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Cloning Vectors
Single stranded vectors are useful

for probe preparation
All of these
for sequencing of cloned DNA
for oligonucleotide directed mutagenesis

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Cloning Vectors
Cos site of the cosmids

both (a) and (b)
consists of 12 bases
contains cleavage site
helps whole genome in circularization and ligation

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MORE MCQ ON Cloning Vectors

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