Cloning Vectors
Cosmids lack

genes coding for viral proteins
origin of replication
marker genes coding for replication
cleavage site for the insertion of foreign DNA

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Cloning Vectors
Phagemid consist of

plasmid vector carrying λ attachment (λ att) site
plasmid vector carrying origin of replication of plasmid only
plasmid vector carrying origin of replication of λ phage only
plasmid vector carrying λ phage's cos site

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Cloning Vectors
Charon vectors are different from EMBL vectors because

physical separation of lambda arm from central fragment is required
they have more extensive range of restriction targets with in their polylinkers.
physical separation of lambda arm from central fragment is not required
both (a) and (b)

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