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Cloning Vectors

Cloning Vectors
Charon 34 and Charon 35 can clone DNA upto

1-2 kb
30-35 kb
6-7 kb
9-20 kb

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Cloning Vectors
Which of the following is not true about phagemid?

Can only be propagated as phage
Contain functional origin of replication of the plasmid and λ phage
Contain λ att site
May be propagated as a plasmid or as phage in appropriate strain

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Cloning Vectors
Cosmid vectors are used for

cloning eukaryotic DNA only
cloning small fragments of DNA
cloning large fragments of DNA
cloning prokaryotic DNA only

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Cloning Vectors
Single stranded vectors are useful

All of these
for probe preparation
for oligonucleotide directed mutagenesis
for sequencing of cloned DNA

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Cloning Vectors
Inserted DNA in λ gt 11 can be expressed as

free protein in the cytoplasm
β-galactosidase fused protein
All of these
free protein that is secreted out

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Cloning Vectors
pBR 322 has/have which of the following selection marker(s)?

Tetr
Ampr
Kanr
Both (a) and (b)

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