Cloning Vectors Maximum size of foreign DNA that can be inserted into a replacement vector is 20-25 kb 18-20 kb 25-30 kb 40-50 kb 20-25 kb 18-20 kb 25-30 kb 40-50 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Which of the following is not true about phagemid? Can only be propagated as phage Contain functional origin of replication of the plasmid and λ phage Contain λ att site May be propagated as a plasmid or as phage in appropriate strain Can only be propagated as phage Contain functional origin of replication of the plasmid and λ phage Contain λ att site May be propagated as a plasmid or as phage in appropriate strain ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors pBR 322 has/have which of the following selection marker(s)? Tetr Both (a) and (b) Ampr Kanr Tetr Both (a) and (b) Ampr Kanr ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors A plasmid can be considered as a suitable cloning vector if it possesses a single restriction site for one or more restriction enzymes All of these it can be readily isolated from the cells insertion of foreign DNA does not alter its replication properties it possesses a single restriction site for one or more restriction enzymes All of these it can be readily isolated from the cells insertion of foreign DNA does not alter its replication properties ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Difference between λ gt 10 and λ gt 11 vectors is that λ gt 10 is a replacement vector λ gt 11 is an expression vector λ gt 10 is an expression vector λ gt 11 is a replacement vector λ gt 10 is a replacement vector λ gt 11 is an expression vector λ gt 10 is an expression vector λ gt 11 is a replacement vector ANSWER DOWNLOAD EXAMIANS APP