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CLONING VECTORS

Cloning Vectors
Maximum size of foreign DNA that can be inserted into a replacement vector is

40-50 kb
18-20 kb
25-30 kb
20-25 kb

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Cloning Vectors
Which of the following is not true about phagemid?

Contain λ att site
Can only be propagated as phage
Contain functional origin of replication of the plasmid and λ phage
May be propagated as a plasmid or as phage in appropriate strain

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Cloning Vectors
pBR 322 has/have which of the following selection marker(s)?

Both (a) and (b)
Kanr
Tetr
Ampr

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Cloning Vectors
A plasmid can be considered as a suitable cloning vector if

it can be readily isolated from the cells
All of these
it possesses a single restriction site for one or more restriction enzymes
insertion of foreign DNA does not alter its replication properties

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Cloning Vectors
Difference between λ gt 10 and λ gt 11 vectors is that

λ gt 10 is a replacement vector
λ gt 10 is an expression vector
λ gt 11 is an expression vector
λ gt 11 is a replacement vector

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