Cloning Vectors A plasmid can be considered as a suitable cloning vector if it possesses a single restriction site for one or more restriction enzymes All of these insertion of foreign DNA does not alter its replication properties it can be readily isolated from the cells it possesses a single restriction site for one or more restriction enzymes All of these insertion of foreign DNA does not alter its replication properties it can be readily isolated from the cells ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Cos site of the cosmids consists of 12 bases both (a) and (b) contains cleavage site helps whole genome in circularization and ligation consists of 12 bases both (a) and (b) contains cleavage site helps whole genome in circularization and ligation ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to 40-44 kb 1-2 kb 20-23 kb 6-7 kb 40-44 kb 1-2 kb 20-23 kb 6-7 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Maximum size of foreign DNA that can be inserted into an insertion vector is 18 kb 27 kb 50 kb 35 kb 18 kb 27 kb 50 kb 35 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Single stranded vectors are useful for probe preparation for sequencing of cloned DNA All of these for oligonucleotide directed mutagenesis for probe preparation for sequencing of cloned DNA All of these for oligonucleotide directed mutagenesis ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Difference between λ gt 10 and λ gt 11 vectors is that λ gt 11 is an expression vector λ gt 10 is a replacement vector λ gt 10 is an expression vector λ gt 11 is a replacement vector λ gt 11 is an expression vector λ gt 10 is a replacement vector λ gt 10 is an expression vector λ gt 11 is a replacement vector ANSWER DOWNLOAD EXAMIANS APP
EXAMIANS