Cloning Vectors A plasmid can be considered as a suitable cloning vector if All of these it can be readily isolated from the cells insertion of foreign DNA does not alter its replication properties it possesses a single restriction site for one or more restriction enzymes All of these it can be readily isolated from the cells insertion of foreign DNA does not alter its replication properties it possesses a single restriction site for one or more restriction enzymes ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Which of the following is not true about phagemid? Contain functional origin of replication of the plasmid and λ phage Contain λ att site May be propagated as a plasmid or as phage in appropriate strain Can only be propagated as phage Contain functional origin of replication of the plasmid and λ phage Contain λ att site May be propagated as a plasmid or as phage in appropriate strain Can only be propagated as phage ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Inserted DNA in λ gt 11 can be expressed as free protein in the cytoplasm All of these β-galactosidase fused protein free protein that is secreted out free protein in the cytoplasm All of these β-galactosidase fused protein free protein that is secreted out ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Stuffer is None of these central fragment of the vector DNA the right arm of the vector DNA the left arm of the vector DNA None of these central fragment of the vector DNA the right arm of the vector DNA the left arm of the vector DNA ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Charon vectors are different from EMBL vectors because physical separation of lambda arm from central fragment is not required both (a) and (b) they have more extensive range of restriction targets with in their polylinkers. physical separation of lambda arm from central fragment is required physical separation of lambda arm from central fragment is not required both (a) and (b) they have more extensive range of restriction targets with in their polylinkers. physical separation of lambda arm from central fragment is required ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Cos site of the cosmids helps whole genome in circularization and ligation both (a) and (b) consists of 12 bases contains cleavage site helps whole genome in circularization and ligation both (a) and (b) consists of 12 bases contains cleavage site ANSWER DOWNLOAD EXAMIANS APP
EXAMIANS