Cloning Vectors A plasmid can be considered as a suitable cloning vector if it can be readily isolated from the cells insertion of foreign DNA does not alter its replication properties it possesses a single restriction site for one or more restriction enzymes All of these it can be readily isolated from the cells insertion of foreign DNA does not alter its replication properties it possesses a single restriction site for one or more restriction enzymes All of these ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Which of the following is not true about phagemid? Contain functional origin of replication of the plasmid and λ phage Contain λ att site May be propagated as a plasmid or as phage in appropriate strain Can only be propagated as phage Contain functional origin of replication of the plasmid and λ phage Contain λ att site May be propagated as a plasmid or as phage in appropriate strain Can only be propagated as phage ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Stuffer is None of these the right arm of the vector DNA the left arm of the vector DNA central fragment of the vector DNA None of these the right arm of the vector DNA the left arm of the vector DNA central fragment of the vector DNA ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Plasmids which are maintained as multiple copy number per cell are known as relaxed plasmids cryptic plasmids none of these stringent plasmids relaxed plasmids cryptic plasmids none of these stringent plasmids ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to 20-23 kb 6-7 kb 40-44 kb 1-2 kb 20-23 kb 6-7 kb 40-44 kb 1-2 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Phagemid consist of plasmid vector carrying origin of replication of plasmid only plasmid vector carrying origin of replication of λ phage only plasmid vector carrying λ phage's cos site plasmid vector carrying λ attachment (λ att) site plasmid vector carrying origin of replication of plasmid only plasmid vector carrying origin of replication of λ phage only plasmid vector carrying λ phage's cos site plasmid vector carrying λ attachment (λ att) site ANSWER DOWNLOAD EXAMIANS APP
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