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Cloning Vectors

Cloning Vectors
Charon 34 and Charon 35 are the examples of

plasmid vector
cosmid vector
phagemid vector
phage vector

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Cloning Vectors
λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to

6-7 kb
20-23 kb
1-2 kb
40-44 kb

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Cloning Vectors
Cos site of the cosmids

consists of 12 bases
helps whole genome in circularization and ligation
both (a) and (b)
contains cleavage site

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Cloning Vectors
EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to

1-2 kb
15-25 kb
6-7 kb
40-44 kb

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Cloning Vectors
Cosmid vectors are used for

cloning eukaryotic DNA only
cloning small fragments of DNA
cloning prokaryotic DNA only
cloning large fragments of DNA

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Cloning Vectors
A plasmid can be considered as a suitable cloning vector if

it possesses a single restriction site for one or more restriction enzymes
insertion of foreign DNA does not alter its replication properties
it can be readily isolated from the cells
All of these

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