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Cloning Vectors

Cloning Vectors
Charon 34 and Charon 35 are the examples of

cosmid vector
phage vector
plasmid vector
phagemid vector

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Cloning Vectors
P1 cloning vector allow cloning of DNA of the length of

20 kbp
50 kbp
100 kbp
10 kbp

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Cloning Vectors
A plasmid can be considered as a suitable cloning vector if

it possesses a single restriction site for one or more restriction enzymes
it can be readily isolated from the cells
insertion of foreign DNA does not alter its replication properties
All of these

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Cloning Vectors
Which of the following is not true about phagemid?

Contain λ att site
Contain functional origin of replication of the plasmid and λ phage
May be propagated as a plasmid or as phage in appropriate strain
Can only be propagated as phage

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Cloning Vectors
Inserted DNA in λ gt 11 can be expressed as

β-galactosidase fused protein
free protein in the cytoplasm
All of these
free protein that is secreted out

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Cloning Vectors
Select the wrong statement about plasmids?

It is extrachromosomal
It is double stranded
It is closed and circular DNA
Its replication depends upon host cell

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