Cloning Vectors
Inserted DNA in λ gt 11 can be expressed as

free protein in the cytoplasm
free protein that is secreted out
All of these
β-galactosidase fused protein

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Cloning Vectors
Phagemid consist of

plasmid vector carrying λ phage's cos site
plasmid vector carrying origin of replication of plasmid only
plasmid vector carrying λ attachment (λ att) site
plasmid vector carrying origin of replication of λ phage only

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Cloning Vectors
Cosmids lack

genes coding for viral proteins
marker genes coding for replication
cleavage site for the insertion of foreign DNA
origin of replication

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