Cloning Vectors Inserted DNA in λ gt 11 can be expressed as free protein in the cytoplasm β-galactosidase fused protein All of these free protein that is secreted out free protein in the cytoplasm β-galactosidase fused protein All of these free protein that is secreted out ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to 40-44 kb 15-25 kb 1-2 kb 6-7 kb 40-44 kb 15-25 kb 1-2 kb 6-7 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Difference between λ gt 10 and λ gt 11 vectors is that λ gt 10 is a replacement vector λ gt 10 is an expression vector λ gt 11 is an expression vector λ gt 11 is a replacement vector λ gt 10 is a replacement vector λ gt 10 is an expression vector λ gt 11 is an expression vector λ gt 11 is a replacement vector ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Maximum size of foreign DNA that can be inserted into an insertion vector is 35 kb 27 kb 18 kb 50 kb 35 kb 27 kb 18 kb 50 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Which of the following is not true about phagemid? May be propagated as a plasmid or as phage in appropriate strain Contain functional origin of replication of the plasmid and λ phage Contain λ att site Can only be propagated as phage May be propagated as a plasmid or as phage in appropriate strain Contain functional origin of replication of the plasmid and λ phage Contain λ att site Can only be propagated as phage ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Charon vectors are different from EMBL vectors because physical separation of lambda arm from central fragment is not required both (a) and (b) they have more extensive range of restriction targets with in their polylinkers. physical separation of lambda arm from central fragment is required physical separation of lambda arm from central fragment is not required both (a) and (b) they have more extensive range of restriction targets with in their polylinkers. physical separation of lambda arm from central fragment is required ANSWER DOWNLOAD EXAMIANS APP
EXAMIANS