Cloning Vectors
Inserted DNA in λ gt 11 can be expressed as

All of these
free protein that is secreted out
β-galactosidase fused protein
free protein in the cytoplasm

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Cloning Vectors
Phagemid consist of

plasmid vector carrying origin of replication of λ phage only
plasmid vector carrying λ phage's cos site
plasmid vector carrying λ attachment (λ att) site
plasmid vector carrying origin of replication of plasmid only

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Cloning Vectors
Cosmids lack

origin of replication
marker genes coding for replication
cleavage site for the insertion of foreign DNA
genes coding for viral proteins

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