Cloning Vectors Phagemid consist of plasmid vector carrying origin of replication of plasmid only plasmid vector carrying origin of replication of λ phage only plasmid vector carrying λ phage's cos site plasmid vector carrying λ attachment (λ att) site plasmid vector carrying origin of replication of plasmid only plasmid vector carrying origin of replication of λ phage only plasmid vector carrying λ phage's cos site plasmid vector carrying λ attachment (λ att) site ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Conjugative plasmids do not exhibit antibiotic resistance exhibit antibiotic resistance carry transfer genes called the tra genes do not carry transfer genes do not exhibit antibiotic resistance exhibit antibiotic resistance carry transfer genes called the tra genes do not carry transfer genes ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Maximum size of foreign DNA that can be inserted into a replacement vector is 20-25 kb 40-50 kb 18-20 kb 25-30 kb 20-25 kb 40-50 kb 18-20 kb 25-30 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Select the wrong statement about plasmids? It is extrachromosomal It is double stranded Its replication depends upon host cell It is closed and circular DNA It is extrachromosomal It is double stranded Its replication depends upon host cell It is closed and circular DNA ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to 40-44 kb 6-7 kb 1-2 kb 15-25 kb 40-44 kb 6-7 kb 1-2 kb 15-25 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors A plasmid can be considered as a suitable cloning vector if insertion of foreign DNA does not alter its replication properties All of these it possesses a single restriction site for one or more restriction enzymes it can be readily isolated from the cells insertion of foreign DNA does not alter its replication properties All of these it possesses a single restriction site for one or more restriction enzymes it can be readily isolated from the cells ANSWER DOWNLOAD EXAMIANS APP
EXAMIANS