Cloning Vectors
Cosmids lack

origin of replication
genes coding for viral proteins
cleavage site for the insertion of foreign DNA
marker genes coding for replication

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Cloning Vectors
Phagemid consist of

plasmid vector carrying λ attachment (λ att) site
plasmid vector carrying λ phage's cos site
plasmid vector carrying origin of replication of λ phage only
plasmid vector carrying origin of replication of plasmid only

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Cloning Vectors
Cosmid vectors are

cryptic plasmids
phages that lack cos site
plasmids that contain fragment of λ DNA including the cos site
plasmids that have no selection marker

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