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Cloning Vectors

Cloning Vectors
λ ZAP vector is an example of

phage
plasmid
cosmid
phagemid

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Cloning Vectors
Maximum size of foreign DNA that can be inserted into a replacement vector is

20-25 kb
40-50 kb
25-30 kb
18-20 kb

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Cloning Vectors
Cryptic plasmids

do not exhibit any phenotypic trait
exhibit one phenotypic traits
exhibit antibiotic resistance
exhibit many phenotypic traits

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Cloning Vectors
Plasmids which are maintained as multiple copy number per cell are known as

none of these
stringent plasmids
relaxed plasmids
cryptic plasmids

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Cloning Vectors
A plasmid can be considered as a suitable cloning vector if

it can be readily isolated from the cells
All of these
insertion of foreign DNA does not alter its replication properties
it possesses a single restriction site for one or more restriction enzymes

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Cloning Vectors
Select the wrong statement about plasmids?

It is double stranded
It is closed and circular DNA
Its replication depends upon host cell
It is extrachromosomal

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