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Cloning Vectors

Cloning Vectors
λ ZAP vector is an example of

phagemid
cosmid
plasmid
phage

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Cloning Vectors
Stuffer is

central fragment of the vector DNA
None of these
the left arm of the vector DNA
the right arm of the vector DNA

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Cloning Vectors
Maximum size of foreign DNA that can be inserted into an insertion vector is

35 kb
27 kb
18 kb
50 kb

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Cloning Vectors
Plasmids which are maintained as multiple copy number per cell are known as

stringent plasmids
relaxed plasmids
none of these
cryptic plasmids

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Cloning Vectors
Cryptic plasmids

exhibit one phenotypic traits
do not exhibit any phenotypic trait
exhibit antibiotic resistance
exhibit many phenotypic traits

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Cloning Vectors
Which of the following is not true about phagemid?

Contain λ att site
Can only be propagated as phage
Contain functional origin of replication of the plasmid and λ phage
May be propagated as a plasmid or as phage in appropriate strain

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