Cloning Vectors
Cos site of the cosmids

helps whole genome in circularization and ligation
contains cleavage site
consists of 12 bases
both (a) and (b)

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Cloning Vectors
Phagemid consist of

plasmid vector carrying λ attachment (λ att) site
plasmid vector carrying origin of replication of plasmid only
plasmid vector carrying origin of replication of λ phage only
plasmid vector carrying λ phage's cos site

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Cloning Vectors
Cosmid vectors are

plasmids that contain fragment of λ DNA including the cos site
phages that lack cos site
cryptic plasmids
plasmids that have no selection marker

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Cloning Vectors
Conjugative plasmids

exhibit antibiotic resistance
carry transfer genes called the tra genes
do not exhibit antibiotic resistance
do not carry transfer genes

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Cloning Vectors
Charon vectors are different from EMBL vectors because

they have more extensive range of restriction targets with in their polylinkers.
physical separation of lambda arm from central fragment is not required
physical separation of lambda arm from central fragment is required
both (a) and (b)

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