Cloning Vectors Cos site of the cosmids both (a) and (b) helps whole genome in circularization and ligation consists of 12 bases contains cleavage site both (a) and (b) helps whole genome in circularization and ligation consists of 12 bases contains cleavage site ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors A plasmid can be considered as a suitable cloning vector if it possesses a single restriction site for one or more restriction enzymes All of these it can be readily isolated from the cells insertion of foreign DNA does not alter its replication properties it possesses a single restriction site for one or more restriction enzymes All of these it can be readily isolated from the cells insertion of foreign DNA does not alter its replication properties ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Which of the following is not true about phagemid? Can only be propagated as phage Contain functional origin of replication of the plasmid and λ phage May be propagated as a plasmid or as phage in appropriate strain Contain λ att site Can only be propagated as phage Contain functional origin of replication of the plasmid and λ phage May be propagated as a plasmid or as phage in appropriate strain Contain λ att site ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors M 13 is an example of both (a) and (b) filamentous phage plasmid single stranded DNA vector both (a) and (b) filamentous phage plasmid single stranded DNA vector ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Cosmid vectors are used for cloning large fragments of DNA cloning prokaryotic DNA only cloning eukaryotic DNA only cloning small fragments of DNA cloning large fragments of DNA cloning prokaryotic DNA only cloning eukaryotic DNA only cloning small fragments of DNA ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Conjugative plasmids do not carry transfer genes exhibit antibiotic resistance do not exhibit antibiotic resistance carry transfer genes called the tra genes do not carry transfer genes exhibit antibiotic resistance do not exhibit antibiotic resistance carry transfer genes called the tra genes ANSWER DOWNLOAD EXAMIANS APP