Cloning Vectors Cos site of the cosmids consists of 12 bases contains cleavage site both (a) and (b) helps whole genome in circularization and ligation consists of 12 bases contains cleavage site both (a) and (b) helps whole genome in circularization and ligation ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to 6-7 kb 1-2 kb 40-44 kb 15-25 kb 6-7 kb 1-2 kb 40-44 kb 15-25 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Plasmid incompatibility is None of these both (a) and (b) inability of a plasmid to grow in the host inability of two different plasmids to coexist in the same host cell in the absence of selection pressure. None of these both (a) and (b) inability of a plasmid to grow in the host inability of two different plasmids to coexist in the same host cell in the absence of selection pressure. ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Which of the following is not true about phagemid? Can only be propagated as phage May be propagated as a plasmid or as phage in appropriate strain Contain λ att site Contain functional origin of replication of the plasmid and λ phage Can only be propagated as phage May be propagated as a plasmid or as phage in appropriate strain Contain λ att site Contain functional origin of replication of the plasmid and λ phage ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors A plasmid can be considered as a suitable cloning vector if insertion of foreign DNA does not alter its replication properties it can be readily isolated from the cells All of these it possesses a single restriction site for one or more restriction enzymes insertion of foreign DNA does not alter its replication properties it can be readily isolated from the cells All of these it possesses a single restriction site for one or more restriction enzymes ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Inserted DNA in λ gt 11 can be expressed as free protein in the cytoplasm β-galactosidase fused protein free protein that is secreted out All of these free protein in the cytoplasm β-galactosidase fused protein free protein that is secreted out All of these ANSWER DOWNLOAD EXAMIANS APP
EXAMIANS