Cloning Vectors
Cos site of the cosmids

helps whole genome in circularization and ligation
consists of 12 bases
both (a) and (b)
contains cleavage site

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Cloning Vectors
Cosmid vectors are

cryptic plasmids
plasmids that contain fragment of λ DNA including the cos site
plasmids that have no selection marker
phages that lack cos site

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Cloning Vectors
Cosmids lack

origin of replication
marker genes coding for replication
cleavage site for the insertion of foreign DNA
genes coding for viral proteins

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Cloning Vectors
Charon vectors are different from EMBL vectors because

they have more extensive range of restriction targets with in their polylinkers.
both (a) and (b)
physical separation of lambda arm from central fragment is not required
physical separation of lambda arm from central fragment is required

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