Cloning Vectors Cosmids lack genes coding for viral proteins marker genes coding for replication origin of replication cleavage site for the insertion of foreign DNA genes coding for viral proteins marker genes coding for replication origin of replication cleavage site for the insertion of foreign DNA ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Which of the following is not true about phagemid? Contain λ att site Can only be propagated as phage Contain functional origin of replication of the plasmid and λ phage May be propagated as a plasmid or as phage in appropriate strain Contain λ att site Can only be propagated as phage Contain functional origin of replication of the plasmid and λ phage May be propagated as a plasmid or as phage in appropriate strain ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Charon vectors are different from EMBL vectors because both (a) and (b) physical separation of lambda arm from central fragment is required they have more extensive range of restriction targets with in their polylinkers. physical separation of lambda arm from central fragment is not required both (a) and (b) physical separation of lambda arm from central fragment is required they have more extensive range of restriction targets with in their polylinkers. physical separation of lambda arm from central fragment is not required ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Cos site of the cosmids both (a) and (b) consists of 12 bases contains cleavage site helps whole genome in circularization and ligation both (a) and (b) consists of 12 bases contains cleavage site helps whole genome in circularization and ligation ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to 1-2 kb 20-23 kb 6-7 kb 40-44 kb 1-2 kb 20-23 kb 6-7 kb 40-44 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Plasmids which are maintained as limited number of copies per cell are known as relaxed plasmids stringent plasmids cryptic plasmids all of these relaxed plasmids stringent plasmids cryptic plasmids all of these ANSWER DOWNLOAD EXAMIANS APP
EXAMIANS