Cloning Vectors
Cosmids lack

marker genes coding for replication
origin of replication
genes coding for viral proteins
cleavage site for the insertion of foreign DNA

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Cloning Vectors
Cosmid vectors are

plasmids that contain fragment of λ DNA including the cos site
plasmids that have no selection marker
cryptic plasmids
phages that lack cos site

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Cloning Vectors
Charon vectors are different from EMBL vectors because

both (a) and (b)
physical separation of lambda arm from central fragment is not required
they have more extensive range of restriction targets with in their polylinkers.
physical separation of lambda arm from central fragment is required

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