Cloning Vectors Cosmids lack marker genes coding for replication genes coding for viral proteins cleavage site for the insertion of foreign DNA origin of replication marker genes coding for replication genes coding for viral proteins cleavage site for the insertion of foreign DNA origin of replication ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Difference between λ gt 10 and λ gt 11 vectors is that λ gt 11 is an expression vector λ gt 11 is a replacement vector λ gt 10 is an expression vector λ gt 10 is a replacement vector λ gt 11 is an expression vector λ gt 11 is a replacement vector λ gt 10 is an expression vector λ gt 10 is a replacement vector ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Inserted DNA in λ gt 11 can be expressed as All of these free protein that is secreted out free protein in the cytoplasm β-galactosidase fused protein All of these free protein that is secreted out free protein in the cytoplasm β-galactosidase fused protein ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to 1-2 kb 40-44 kb 6-7 kb 20-23 kb 1-2 kb 40-44 kb 6-7 kb 20-23 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Which of the following is not true about phagemid? May be propagated as a plasmid or as phage in appropriate strain Can only be propagated as phage Contain λ att site Contain functional origin of replication of the plasmid and λ phage May be propagated as a plasmid or as phage in appropriate strain Can only be propagated as phage Contain λ att site Contain functional origin of replication of the plasmid and λ phage ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Charon vectors are different from EMBL vectors because both (a) and (b) they have more extensive range of restriction targets with in their polylinkers. physical separation of lambda arm from central fragment is not required physical separation of lambda arm from central fragment is required both (a) and (b) they have more extensive range of restriction targets with in their polylinkers. physical separation of lambda arm from central fragment is not required physical separation of lambda arm from central fragment is required ANSWER DOWNLOAD EXAMIANS APP
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