Cloning Vectors
Single stranded vectors are useful

for oligonucleotide directed mutagenesis
for sequencing of cloned DNA
for probe preparation
All of these

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Cloning Vectors
Cosmids lack

cleavage site for the insertion of foreign DNA
origin of replication
marker genes coding for replication
genes coding for viral proteins

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Cloning Vectors
Cosmid vectors are

plasmids that have no selection marker
phages that lack cos site
plasmids that contain fragment of λ DNA including the cos site
cryptic plasmids

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