Cloning Vectors Single stranded vectors are useful for sequencing of cloned DNA All of these for probe preparation for oligonucleotide directed mutagenesis for sequencing of cloned DNA All of these for probe preparation for oligonucleotide directed mutagenesis ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Which of the following is not true about phagemid? Contain λ att site May be propagated as a plasmid or as phage in appropriate strain Contain functional origin of replication of the plasmid and λ phage Can only be propagated as phage Contain λ att site May be propagated as a plasmid or as phage in appropriate strain Contain functional origin of replication of the plasmid and λ phage Can only be propagated as phage ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Maximum size of foreign DNA that can be inserted into an insertion vector is 35 kb 27 kb 18 kb 50 kb 35 kb 27 kb 18 kb 50 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Inserted DNA in λ gt 11 can be expressed as All of these β-galactosidase fused protein free protein in the cytoplasm free protein that is secreted out All of these β-galactosidase fused protein free protein in the cytoplasm free protein that is secreted out ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to 20-23 kb 1-2 kb 6-7 kb 40-44 kb 20-23 kb 1-2 kb 6-7 kb 40-44 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Cosmid vectors are used for cloning small fragments of DNA cloning large fragments of DNA cloning prokaryotic DNA only cloning eukaryotic DNA only cloning small fragments of DNA cloning large fragments of DNA cloning prokaryotic DNA only cloning eukaryotic DNA only ANSWER DOWNLOAD EXAMIANS APP
EXAMIANS