Cloning Vectors
Single stranded vectors are useful

for oligonucleotide directed mutagenesis
for sequencing of cloned DNA
for probe preparation
All of these

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Cloning Vectors
Cosmid vectors are

phages that lack cos site
plasmids that contain fragment of λ DNA including the cos site
cryptic plasmids
plasmids that have no selection marker

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Cloning Vectors
Conjugative plasmids

carry transfer genes called the tra genes
do not exhibit antibiotic resistance
exhibit antibiotic resistance
do not carry transfer genes

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