Cloning Vectors Which of the following is not true about phagemid? Can only be propagated as phage Contain functional origin of replication of the plasmid and λ phage Contain λ att site May be propagated as a plasmid or as phage in appropriate strain Can only be propagated as phage Contain functional origin of replication of the plasmid and λ phage Contain λ att site May be propagated as a plasmid or as phage in appropriate strain ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Single stranded vectors are useful for probe preparation for sequencing of cloned DNA All of these for oligonucleotide directed mutagenesis for probe preparation for sequencing of cloned DNA All of these for oligonucleotide directed mutagenesis ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors pBR 322 has/have which of the following selection marker(s)? Ampr Both (a) and (b) Tetr Kanr Ampr Both (a) and (b) Tetr Kanr ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Inserted DNA in λ gt 11 can be expressed as free protein that is secreted out free protein in the cytoplasm β-galactosidase fused protein All of these free protein that is secreted out free protein in the cytoplasm β-galactosidase fused protein All of these ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Cosmid vectors are used for cloning large fragments of DNA cloning small fragments of DNA cloning eukaryotic DNA only cloning prokaryotic DNA only cloning large fragments of DNA cloning small fragments of DNA cloning eukaryotic DNA only cloning prokaryotic DNA only ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Maximum size of foreign DNA that can be inserted into an insertion vector is 50 kb 35 kb 18 kb 27 kb 50 kb 35 kb 18 kb 27 kb ANSWER DOWNLOAD EXAMIANS APP