Cloning Vectors Which of the following is not true about phagemid? Contain functional origin of replication of the plasmid and λ phage Contain λ att site Can only be propagated as phage May be propagated as a plasmid or as phage in appropriate strain Contain functional origin of replication of the plasmid and λ phage Contain λ att site Can only be propagated as phage May be propagated as a plasmid or as phage in appropriate strain ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors pBR 322 has/have which of the following selection marker(s)? Kanr Tetr Ampr Both (a) and (b) Kanr Tetr Ampr Both (a) and (b) ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Charon vectors are different from EMBL vectors because they have more extensive range of restriction targets with in their polylinkers. physical separation of lambda arm from central fragment is not required physical separation of lambda arm from central fragment is required both (a) and (b) they have more extensive range of restriction targets with in their polylinkers. physical separation of lambda arm from central fragment is not required physical separation of lambda arm from central fragment is required both (a) and (b) ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Maximum size of foreign DNA that can be inserted into a replacement vector is 18-20 kb 40-50 kb 20-25 kb 25-30 kb 18-20 kb 40-50 kb 20-25 kb 25-30 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Select the wrong statement about plasmids? Its replication depends upon host cell It is double stranded It is extrachromosomal It is closed and circular DNA Its replication depends upon host cell It is double stranded It is extrachromosomal It is closed and circular DNA ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Cosmid vectors are plasmids that have no selection marker phages that lack cos site plasmids that contain fragment of λ DNA including the cos site cryptic plasmids plasmids that have no selection marker phages that lack cos site plasmids that contain fragment of λ DNA including the cos site cryptic plasmids ANSWER DOWNLOAD EXAMIANS APP