Cloning Vectors Which of the following is not true about phagemid? Contain functional origin of replication of the plasmid and λ phage Can only be propagated as phage Contain λ att site May be propagated as a plasmid or as phage in appropriate strain Contain functional origin of replication of the plasmid and λ phage Can only be propagated as phage Contain λ att site May be propagated as a plasmid or as phage in appropriate strain ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Cos site of the cosmids consists of 12 bases both (a) and (b) contains cleavage site helps whole genome in circularization and ligation consists of 12 bases both (a) and (b) contains cleavage site helps whole genome in circularization and ligation ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Plasmid incompatibility is None of these both (a) and (b) inability of two different plasmids to coexist in the same host cell in the absence of selection pressure. inability of a plasmid to grow in the host None of these both (a) and (b) inability of two different plasmids to coexist in the same host cell in the absence of selection pressure. inability of a plasmid to grow in the host ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Maximum size of foreign DNA that can be inserted into a replacement vector is 18-20 kb 25-30 kb 20-25 kb 40-50 kb 18-20 kb 25-30 kb 20-25 kb 40-50 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Size of the DNA that can be packaged into a λ phage is 40-50 kb 35-53 kb any size 50 kb 40-50 kb 35-53 kb any size 50 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Select the wrong statement about plasmids? It is extrachromosomal It is closed and circular DNA Its replication depends upon host cell It is double stranded It is extrachromosomal It is closed and circular DNA Its replication depends upon host cell It is double stranded ANSWER DOWNLOAD EXAMIANS APP