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Cloning Vectors

Cloning Vectors
Maximum size of foreign DNA that can be inserted into an insertion vector is

35 kb
27 kb
18 kb
50 kb

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Cloning Vectors
A plasmid can be considered as a suitable cloning vector if

it possesses a single restriction site for one or more restriction enzymes
it can be readily isolated from the cells
All of these
insertion of foreign DNA does not alter its replication properties

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Cloning Vectors
Single stranded vectors are useful

for oligonucleotide directed mutagenesis
for probe preparation
All of these
for sequencing of cloned DNA

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Cloning Vectors
P1 cloning vector is the example of

bacteriophage
plasmid
phagemid
cosmid

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Cloning Vectors
Cos site of the cosmids

helps whole genome in circularization and ligation
consists of 12 bases
both (a) and (b)
contains cleavage site

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Cloning Vectors
P1 cloning vector allow cloning of DNA of the length of

10 kbp
50 kbp
100 kbp
20 kbp

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MORE MCQ ON Cloning Vectors

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