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Cloning Vectors

Cloning Vectors
Maximum size of foreign DNA that can be inserted into an insertion vector is

18 kb
35 kb
50 kb
27 kb

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Cloning Vectors
Size of the DNA that can be packaged into a λ phage is

40-50 kb
50 kb
any size
35-53 kb

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Cloning Vectors
Plasmids which are maintained as multiple copy number per cell are known as

relaxed plasmids
stringent plasmids
none of these
cryptic plasmids

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Cloning Vectors
Charon 34 and Charon 35 are the examples of

plasmid vector
cosmid vector
phagemid vector
phage vector

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Cloning Vectors
P1 cloning vector is the example of

cosmid
bacteriophage
plasmid
phagemid

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Cloning Vectors
Which of the following is not true about phagemid?

Contain functional origin of replication of the plasmid and λ phage
Contain λ att site
Can only be propagated as phage
May be propagated as a plasmid or as phage in appropriate strain

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