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Cloning Vectors

Cloning Vectors
Maximum size of foreign DNA that can be inserted into a replacement vector is

25-30 kb
20-25 kb
40-50 kb
18-20 kb

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Cloning Vectors
Plasmids which are maintained as limited number of copies per cell are known as

cryptic plasmids
stringent plasmids
all of these
relaxed plasmids

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Cloning Vectors
Single stranded vectors are useful

for oligonucleotide directed mutagenesis
for sequencing of cloned DNA
for probe preparation
All of these

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Cloning Vectors
Cos site of the cosmids

contains cleavage site
consists of 12 bases
helps whole genome in circularization and ligation
both (a) and (b)

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Cloning Vectors
Select the wrong statement about plasmids?

It is double stranded
It is extrachromosomal
Its replication depends upon host cell
It is closed and circular DNA

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Cloning Vectors
Which of the following is not true about phagemid?

Can only be propagated as phage
May be propagated as a plasmid or as phage in appropriate strain
Contain functional origin of replication of the plasmid and λ phage
Contain λ att site

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