Cloning Vectors Plasmid incompatibility is inability of a plasmid to grow in the host both (a) and (b) inability of two different plasmids to coexist in the same host cell in the absence of selection pressure. None of these inability of a plasmid to grow in the host both (a) and (b) inability of two different plasmids to coexist in the same host cell in the absence of selection pressure. None of these ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors λ ZAP vector is an example of cosmid phagemid phage plasmid cosmid phagemid phage plasmid ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Which of the following is not true about phagemid? Can only be propagated as phage May be propagated as a plasmid or as phage in appropriate strain Contain functional origin of replication of the plasmid and λ phage Contain λ att site Can only be propagated as phage May be propagated as a plasmid or as phage in appropriate strain Contain functional origin of replication of the plasmid and λ phage Contain λ att site ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to 40-44 kb 1-2 kb 15-25 kb 6-7 kb 40-44 kb 1-2 kb 15-25 kb 6-7 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors A plasmid can be considered as a suitable cloning vector if it can be readily isolated from the cells insertion of foreign DNA does not alter its replication properties All of these it possesses a single restriction site for one or more restriction enzymes it can be readily isolated from the cells insertion of foreign DNA does not alter its replication properties All of these it possesses a single restriction site for one or more restriction enzymes ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Maximum size of foreign DNA that can be inserted into an insertion vector is 35 kb 18 kb 27 kb 50 kb 35 kb 18 kb 27 kb 50 kb ANSWER DOWNLOAD EXAMIANS APP