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Cloning Vectors

Cloning Vectors
P1 cloning vector is the example of

bacteriophage
cosmid
phagemid
plasmid

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Cloning Vectors
Cosmid vectors are used for

cloning prokaryotic DNA only
cloning small fragments of DNA
cloning eukaryotic DNA only
cloning large fragments of DNA

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Cloning Vectors
Maximum size of foreign DNA that can be inserted into a replacement vector is

18-20 kb
40-50 kb
25-30 kb
20-25 kb

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Cloning Vectors
Stuffer is

central fragment of the vector DNA
the right arm of the vector DNA
None of these
the left arm of the vector DNA

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Cloning Vectors
Difference between λ gt 10 and λ gt 11 vectors is that

λ gt 10 is an expression vector
λ gt 11 is an expression vector
λ gt 10 is a replacement vector
λ gt 11 is a replacement vector

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Cloning Vectors
Which of the following is not true about phagemid?

May be propagated as a plasmid or as phage in appropriate strain
Contain functional origin of replication of the plasmid and λ phage
Contain λ att site
Can only be propagated as phage

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