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Cloning Vectors

Cloning Vectors
P1 cloning vector is the example of

plasmid
phagemid
cosmid
bacteriophage

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Cloning Vectors
Maximum size of foreign DNA that can be inserted into an insertion vector is

18 kb
35 kb
27 kb
50 kb

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Cloning Vectors
Inserted DNA in λ gt 11 can be expressed as

All of these
free protein in the cytoplasm
free protein that is secreted out
β-galactosidase fused protein

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Cloning Vectors
Cos site of the cosmids

consists of 12 bases
both (a) and (b)
helps whole genome in circularization and ligation
contains cleavage site

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Cloning Vectors
Cosmid vectors are used for

cloning small fragments of DNA
cloning prokaryotic DNA only
cloning large fragments of DNA
cloning eukaryotic DNA only

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Cloning Vectors
λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to

1-2 kb
40-44 kb
6-7 kb
20-23 kb

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MORE MCQ ON Cloning Vectors

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