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Cloning Vectors

Cloning Vectors
pBR 322 has/have which of the following selection marker(s)?

Tetr
Ampr
Kanr
Both (a) and (b)

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Cloning Vectors
Maximum size of foreign DNA that can be inserted into a replacement vector is

25-30 kb
40-50 kb
20-25 kb
18-20 kb

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Cloning Vectors
Inserted DNA in λ gt 11 can be expressed as

All of these
free protein that is secreted out
β-galactosidase fused protein
free protein in the cytoplasm

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Cloning Vectors
Cos site of the cosmids

both (a) and (b)
contains cleavage site
helps whole genome in circularization and ligation
consists of 12 bases

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Cloning Vectors
P1 cloning vector is the example of

phagemid
cosmid
plasmid
bacteriophage

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Cloning Vectors
λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to

6-7 kb
1-2 kb
40-44 kb
20-23 kb

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