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Cloning Vectors

Cloning Vectors
pBR 322 has/have which of the following selection marker(s)?

Tetr
Kanr
Both (a) and (b)
Ampr

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Cloning Vectors
Phagemid vectors are

combination of phages and cosmid
All of these
phages carrying properties of plasmids
combination of plasmid and phage λ

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Cloning Vectors
A plasmid can be considered as a suitable cloning vector if

insertion of foreign DNA does not alter its replication properties
it possesses a single restriction site for one or more restriction enzymes
it can be readily isolated from the cells
All of these

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Cloning Vectors
Select the wrong statement about plasmids?

It is extrachromosomal
It is closed and circular DNA
It is double stranded
Its replication depends upon host cell

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Cloning Vectors
M 13 is an example of

filamentous phage
both (a) and (b)
plasmid
single stranded DNA vector

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Cloning Vectors
EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to

1-2 kb
6-7 kb
15-25 kb
40-44 kb

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