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Cloning Vectors

Cloning Vectors
pBR 322 has/have which of the following selection marker(s)?

Ampr
Both (a) and (b)
Kanr
Tetr

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Cloning Vectors
Size of the DNA that can be packaged into a λ phage is

50 kb
any size
35-53 kb
40-50 kb

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Cloning Vectors
Cosmid vectors are used for

cloning prokaryotic DNA only
cloning small fragments of DNA
cloning eukaryotic DNA only
cloning large fragments of DNA

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Cloning Vectors
Difference between λ gt 10 and λ gt 11 vectors is that

λ gt 10 is an expression vector
λ gt 11 is a replacement vector
λ gt 11 is an expression vector
λ gt 10 is a replacement vector

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Cloning Vectors
Maximum size of foreign DNA that can be inserted into a replacement vector is

40-50 kb
20-25 kb
18-20 kb
25-30 kb

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Cloning Vectors
Which of the following is not true about phagemid?

Contain λ att site
Can only be propagated as phage
May be propagated as a plasmid or as phage in appropriate strain
Contain functional origin of replication of the plasmid and λ phage

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