Cloning Vectors pBR 322 has/have which of the following selection marker(s)? Ampr Both (a) and (b) Tetr Kanr Ampr Both (a) and (b) Tetr Kanr ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Maximum size of foreign DNA that can be inserted into a replacement vector is 25-30 kb 20-25 kb 18-20 kb 40-50 kb 25-30 kb 20-25 kb 18-20 kb 40-50 kb ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Which of the following is not true about phagemid? Contain λ att site May be propagated as a plasmid or as phage in appropriate strain Contain functional origin of replication of the plasmid and λ phage Can only be propagated as phage Contain λ att site May be propagated as a plasmid or as phage in appropriate strain Contain functional origin of replication of the plasmid and λ phage Can only be propagated as phage ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors A plasmid can be considered as a suitable cloning vector if it possesses a single restriction site for one or more restriction enzymes insertion of foreign DNA does not alter its replication properties it can be readily isolated from the cells All of these it possesses a single restriction site for one or more restriction enzymes insertion of foreign DNA does not alter its replication properties it can be readily isolated from the cells All of these ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Plasmids which are maintained as limited number of copies per cell are known as cryptic plasmids all of these stringent plasmids relaxed plasmids cryptic plasmids all of these stringent plasmids relaxed plasmids ANSWER DOWNLOAD EXAMIANS APP
Cloning Vectors Size of the DNA that can be packaged into a λ phage is any size 50 kb 35-53 kb 40-50 kb any size 50 kb 35-53 kb 40-50 kb ANSWER DOWNLOAD EXAMIANS APP