Recombinant DNA Technology
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

PCR-amplified variable numbers of tandem repeats (VNTRs)
copies of the gene with an altered sequence so that a restriction site is inserted
mutagenized copies of a gene
short sequences that will hybridize only to a specific base sequence

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Recombinant DNA Technology
A human cDNA library

None of these
cannot be used to obtain human genes because it would be radioactive
contains DNA for virtually all of the human proteins in vectors
contains DNA for specific human proteins

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