Recombinant DNA Technology
Knockout mice are created by

infecting the mouse with a retrovirus
mutagenizing a mouse and selecting for mutant offspring
creating a chimera by fusing cells from two different cell lines
transfecting embryonic stem cells with an altered gene sequence

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Recombinant DNA Technology
An expression vector

always contains DNA segments for the regulation of mRNA production
usually contains a gene that confers antibiotic resistance to the bacterial host
All of these
always contains an origin of replication

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Recombinant DNA Technology
An example of a restriction fragment length polymorphism is

All of these
cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved
different length fragments of DNA resulting from loss or gain of a restriction site
an Eco RI cuts DNA at a different sequence than Hind III

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