Recombinant DNA Technology
Which of the following is correct in terms of determination of location of genetic traits?

Known protein coding sequences are too far apart to allow linkage determination for most new genes
Protein-coding genes are always associated with a restriction pattern
None of these
Restriction sites allow DNAs to be digested

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Recombinant DNA Technology
Knockout mice are created by

mutagenizing a mouse and selecting for mutant offspring
infecting the mouse with a retrovirus
creating a chimera by fusing cells from two different cell lines
transfecting embryonic stem cells with an altered gene sequence

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Recombinant DNA Technology
The order for the construction of a cDNA fragment from mRNA is to

bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase
treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase

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