Recombinant DNA Technology
Isoschizomers recognize

same recognition site and different recognition sequence
same recognition sequence but different recognition site
same recognition site and recognition sequence
different recognition site and different recognition sequence

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Recombinant DNA Technology
An expression vector

All of these
always contains DNA segments for the regulation of mRNA production
usually contains a gene that confers antibiotic resistance to the bacterial host
always contains an origin of replication

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Recombinant DNA Technology
The order for the construction of a cDNA fragment from mRNA is to

digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase
treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase

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Recombinant DNA Technology
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

copies of the gene with an altered sequence so that a restriction site is inserted
mutagenized copies of a gene
PCR-amplified variable numbers of tandem repeats (VNTRs)
short sequences that will hybridize only to a specific base sequence

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