Recombinant DNA Technology
Knockout mice are created by

infecting the mouse with a retrovirus
creating a chimera by fusing cells from two different cell lines
mutagenizing a mouse and selecting for mutant offspring
transfecting embryonic stem cells with an altered gene sequence

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Recombinant DNA Technology
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

short sequences that will hybridize only to a specific base sequence
copies of the gene with an altered sequence so that a restriction site is inserted
mutagenized copies of a gene
PCR-amplified variable numbers of tandem repeats (VNTRs)

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Recombinant DNA Technology
Which of the following is correct in terms of determination of location of genetic traits?

Restriction sites allow DNAs to be digested
Protein-coding genes are always associated with a restriction pattern
Known protein coding sequences are too far apart to allow linkage determination for most new genes
None of these

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Recombinant DNA Technology
An expression vector

always contains DNA segments for the regulation of mRNA production
usually contains a gene that confers antibiotic resistance to the bacterial host
All of these
always contains an origin of replication

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