Recombinant DNA Technology
Isoschizomers recognize

different recognition site and different recognition sequence
same recognition site and recognition sequence
same recognition site and different recognition sequence
same recognition sequence but different recognition site

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Recombinant DNA Technology
The order for the construction of a cDNA fragment from mRNA is to

digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase
treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase

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Recombinant DNA Technology
An example of a restriction fragment length polymorphism is

an Eco RI cuts DNA at a different sequence than Hind III
All of these
different length fragments of DNA resulting from loss or gain of a restriction site
cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved

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Recombinant DNA Technology
Knockout mice are created by

mutagenizing a mouse and selecting for mutant offspring
infecting the mouse with a retrovirus
transfecting embryonic stem cells with an altered gene sequence
creating a chimera by fusing cells from two different cell lines

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